核糖体的发育及核糖体核糖核酸的甲基化
Ribosome development and the methylation of ribosomal ribonucleic acid.
作者信息
Sypherd P S
出版信息
J Bacteriol. 1968 May;95(5):1844-50. doi: 10.1128/jb.95.5.1844-1850.1968.
Abstract
Immature ribonucleoprotein particles accumulate in amino acid-starved cells of a relaxed mutant of Escherichia coli. The ribonucleic acid (RNA) of these particles is nonmethylated in cells starved for methionine. However, in bacteria starved for arginine, lysine, or histidine, the RNA of these particles is one-half methylated. The relationship of submethylation to a structural alteration in the same RNA was studied. The results of kinetic studies showed that submethylation and the structural transition are not causally related, since they are described by different rate constants. Moreover, it was possible to accumulate fully methylated immature-particle RNA that possessed the sedimentation and chromatographic properties of nonmethylated RNA. It was concluded that, during the normal course of ribosome development, methylation of ribosomal RNA is completed prior to the final maturation steps.
摘要
未成熟的核糖核蛋白颗粒在大肠杆菌松弛型突变体的氨基酸饥饿细胞中积累。在缺乏甲硫氨酸的细胞中，这些颗粒的核糖核酸（RNA）未被甲基化。然而，在缺乏精氨酸、赖氨酸或组氨酸的细菌中，这些颗粒的RNA有一半被甲基化。研究了亚甲基化与同一RNA结构改变的关系。动力学研究结果表明，亚甲基化与结构转变没有因果关系，因为它们由不同的速率常数描述。此外，有可能积累具有非甲基化RNA沉降和色谱性质的完全甲基化的未成熟颗粒RNA。得出的结论是，在核糖体正常发育过程中，核糖体RNA的甲基化在最终成熟步骤之前完成。
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