Paynter M J, Hungate R E
J Bacteriol. 1968 May;95(5):1943-51. doi: 10.1128/jb.95.5.1943-1951.1968.
A methanogenic bacterium, present in bovine rumen contents at concentrations of approximately 2 x 10(8) cells per ml, has been isolated in pure culture. The organism is a strictly anaerobic, weakly motile, nonsporeforming, gram-negative rod (0.7 mu x 1.5 to 2.0 mu) with rounded ends. There is a single polar flagellum. The organism grows at temperatures between 30 and 45 C, with an optimum at 40 C, and at pH values between 5.9 and 7.7, with optimal growth between pH 6.1 and 6.9. Of the 17 substrates tested, only formate and H(2) plus CO(2) supported growth. An unidentified, heat-stable factor(s) was required by the organism. The factor, which was not one of the common ones, was present in rumen fluid, mixed rumen bacteria, and yeast extract. On the basis of colony morphology, Gram reaction, and motility, the organism is classified as a new species of methanogenic bacterium, and the name Methanobacterium mobilis sp. n. is proposed.
一种产甲烷细菌已被纯培养分离出来，它存在于牛瘤胃液中，浓度约为每毫升2×10⁸个细胞。该微生物是严格厌氧、运动性弱、不产芽孢、革兰氏阴性的杆菌（0.7微米×1.5至2.0微米），两端圆形。有一根单极鞭毛。该微生物在30至45℃之间生长，最适温度为40℃，在pH值5.9至7.7之间生长，最适生长pH值在6.1至6.9之间。在测试的17种底物中，只有甲酸盐和H₂加CO₂能支持其生长。该微生物需要一种未鉴定的热稳定因子。这种因子不是常见因子之一，存在于瘤胃液、混合瘤胃细菌和酵母提取物中。根据菌落形态、革兰氏反应和运动性，该微生物被归类为产甲烷细菌的一个新种，并提出名为运动产甲烷杆菌（Methanobacterium mobilis sp. n.）。
