Moyer J A, O'Donohue T L, Herrenkohl L R, Gala R R, Jacobowitz D M
Brain Res. 1979 Oct 26;176(1):125-33. doi: 10.1016/0006-8993(79)90874-6.
The effects of suckling on serum prolactin levels and catecholamine concentrations and turnover were examined in several discrete brain regions. Turnover rates were assessed by using the synthesis inhibitor alpha-methyltyrosine (alpha-MT) in combination with microdissection techniques for the removal of individual brain regions and sensitive radioenzymatic assays for norepinephrine (NE) and dopaime (DA). Prolactin secretion was induced by mothers experiencing 6 h of pup removal with subsequent pup replacement. Suckling or the administration of alpha-MT to mothers resulted in a marked increase in circulating titers of prolactin. A decrease in steady-state NE concentrations in the anterior hypothalamus and a decrease in steady-state DA concentrations in the ventromedial nucleus were noted in suckled mothers. The comparison of relative rates of NE depletion after alpha-MT treatment revealed a suckling-induced increase in turnover in the ventromedial nucleus and a suckling-induced decrease in turnover in the anterior hypothalamus. Neither suckling nor alpha-MT treatment produced any changes in NE or DA turnover rates in the arcuate nucleus or median eminence. These findings demonstrate that suckling-induced activation of prolactin results in changes in noradrenergic processes in the ventromedial and anterior hypothalamic nuclei. This suggests an involvement of noradrenergic systems in suckling-induced prolactin release.
在几个离散的脑区中研究了哺乳对血清催乳素水平、儿茶酚胺浓度及周转率的影响。通过使用合成抑制剂α-甲基酪氨酸（α-MT）结合显微解剖技术去除各个脑区，并采用灵敏的放射性酶法测定去甲肾上腺素（NE）和多巴胺（DA）来评估周转率。通过让母鼠经历6小时的幼崽移除并随后替换幼崽来诱导催乳素分泌。对母鼠进行哺乳或给予α-MT导致循环中的催乳素滴度显著增加。在哺乳的母鼠中，观察到下丘脑前部的稳态NE浓度降低，腹内侧核的稳态DA浓度降低。对α-MT处理后NE消耗相对速率的比较显示，哺乳诱导腹内侧核周转率增加，而哺乳诱导下丘脑前部周转率降低。哺乳和α-MT处理均未引起弓状核或正中隆起中NE或DA周转率的任何变化。这些发现表明，哺乳诱导的催乳素激活导致腹内侧和下丘脑前部核团中去甲肾上腺素能过程的变化。这表明去甲肾上腺素能系统参与了哺乳诱导的催乳素释放。
