Owen J A, Nakatsu K
Clin Chem. 1978 Feb;24(2):367-8.
A spectrophotometric assay procedure is described for individually measuring theophylline-7-acetic acid and theophylline in 2.0 ml of serum. Absorbance is a linear function of concentration over a range of 0.5-40 mg/liter for theophylline-7-acetic acid and 0.5-40 mg/liter for theophylline. No endogenous or exogenous compounds were found that interfere with theophylline-7-acetic acid determinations. As with other spectrophotometric theophylline assays, theobromine and, to a slight extent, phenobarbital interfere with theophylline determinations.
本文描述了一种分光光度测定法，用于单独测定2.0 ml血清中的7-乙酸茶碱和茶碱。在0.5 - 40 mg/升的浓度范围内，吸光度与7-乙酸茶碱和茶碱的浓度呈线性关系。未发现有内源性或外源性化合物干扰7-乙酸茶碱的测定。与其他分光光度法测定茶碱一样，可可碱以及在一定程度上苯巴比妥会干扰茶碱的测定。
