Amsterdam D, Wolfe M W
Appl Microbiol. 1968 Oct;16(10):1460-4. doi: 10.1128/am.16.10.1460-1464.1968.
The means for distinguishing Escherichia from Aerobacter (Enterobacter) differ in laboratories and range from complete dependence on colonial reactions on typical gram-negative media to reliance on one or more of the classical indole, methyl red, Voges-Proskauer, citrate (IMViC) parameters. Three colonial types (one prejudged as Escherichia) of lactose-positive rods were catalogued on each of the most commonly used selective media, MacConkey Agar, Endo Agar, and E M B Agar. Each cultural type was presumptively diagnosed and then compared with the expected outcome of individual IMViC tests. The distribution of preliminary identifications was similar from growth patterns on MacConkey Agar and E M B Agar, but it differed markedly from Endo Agar. When organisms initially diagnosed by cultural methods were compared by single IMViC tests, it was found that for each colonial type one of the biochemical parameters was best suited. Thus, for those types initially considered Escherichia, the methyl red or Voges-Proskauer test results agreed most consistently; for other types, the citrate reaction was most satisfactory. In addition, when newly formulated reagent-impregnated paper strip methods for indole, Voges-Proskauer, and citrate were evaluated and compared to the standard methods, agreement was 97% for indole, 90% for Voges-Proskauer, and 95% for Simmons' citrate.
区分大肠杆菌与气杆菌（肠杆菌）的方法在不同实验室有所不同，范围从完全依赖典型革兰氏阴性培养基上的菌落反应到依赖一种或多种经典的吲哚、甲基红、Voges-Proskauer、柠檬酸盐（IMViC）参数。在最常用的选择性培养基，即麦康凯琼脂、远藤琼脂和伊红美蓝琼脂上，对乳糖阳性杆菌的三种菌落类型（其中一种预先判定为大肠杆菌）进行了分类。对每种培养类型进行初步诊断，然后与各个IMViC试验的预期结果进行比较。麦康凯琼脂和伊红美蓝琼脂上生长模式的初步鉴定分布相似，但与远藤琼脂上的结果明显不同。当通过单一IMViC试验对最初通过培养方法诊断的微生物进行比较时，发现对于每种菌落类型，其中一个生化参数最为适用。因此，对于那些最初被认为是大肠杆菌的类型，甲基红或Voges-Proskauer试验结果最为一致；对于其他类型，柠檬酸盐反应最为令人满意。此外，当对新配制的用于吲哚、Voges-Proskauer和柠檬酸盐的试剂浸渍纸条方法进行评估并与标准方法进行比较时，吲哚的一致性为97%，Voges-Proskauer为90%，西蒙斯柠檬酸盐为95%。
