Lubaroff D M, Waksman B H
J Exp Med. 1968 Dec 1;128(6):1437-47. doi: 10.1084/jem.128.6.1437.
The precise origin of cells infiltrating tuberculin skin reactions was studied with the technique of immunofluorescence. Thymectomized, irradiated Lewis rats were restored with bone marrow from allogeneic or F(1) donors. They were passively sensitized to tuberculin by a subsequent transfer of Lewis lymph node cells and were given intradermal skin tests with tuberculoprotein. In 24 hr reactions the majority of cells were shown to be derived from the infused marrow. These results were the same regardless whether the lymphocyte transfer was performed on the day of irradiation and marrow injection or 7 days later. The cells in the tuberculin reactions, marrow, spleen, and lymph nodes not derived from the bone marrow were found to originate in the transferred lymph node cells. The relative percentages of marrow-derived and lymph node-derived cells in the tuberculin reactions remained the same during the 9-24 hr period following skin test.
运用免疫荧光技术研究了浸润结核菌素皮肤反应的细胞的确切来源。对经胸腺切除、照射过的Lewis大鼠，用来自同种异体或F(1)供体的骨髓进行恢复。通过随后转移Lewis淋巴结细胞使它们被动致敏于结核菌素，并对其进行结核蛋白的皮内皮肤试验。在24小时的反应中，大多数细胞显示来源于注入的骨髓。无论淋巴细胞转移是在照射和骨髓注射当天进行还是在7天后进行，结果都是相同的。发现在结核菌素反应、骨髓、脾脏和淋巴结中并非来源于骨髓的细胞起源于转移的淋巴结细胞。在皮肤试验后的9至24小时期间，结核菌素反应中骨髓来源和淋巴结来源细胞的相对百分比保持不变。
