大肠杆菌中磷酸转移酶突变体的分离与定位
Isolation and mapping of phosphotransferase mutants in Escherichia coli.
作者信息
Epstein W, Jewett S, Fox C F
出版信息
J Bacteriol. 1970 Nov;104(2):793-7. doi: 10.1128/jb.104.2.793-797.1970.
Abstract
Mutants of Escherichia coli K-12 defective in enzyme I or Hpr, the two common components of the phosphoenolpyruvate-dependent phosphotransferase system, were isolated by a simple, direct method. The ptsI locus, the structural gene for enzyme I, and the ptsH locus, the site of mutations leading to loss of Hpr activity, are adjacent genes and could be part of a single operon. These two genes lie between the purC and supN markers in the order: strA... guaB-purC-ptsI-ptsH-supN-dsdA... his.
摘要
通过一种简单、直接的方法分离出了大肠杆菌K-12中磷酸烯醇丙酮酸依赖性磷酸转移酶系统的两个常见组分即酶I或Hpr有缺陷的突变体。酶I的结构基因ptsI位点和导致Hpr活性丧失的突变位点ptsH位点是相邻基因，可能是单个操纵子的一部分。这两个基因按以下顺序位于purC和supN标记之间：strA...guaB-purC-ptsI-ptsH-supN-dsdA...his。
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