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大肠杆菌中磷酸转移酶突变体的分离与定位

Isolation and mapping of phosphotransferase mutants in Escherichia coli.

作者信息

Epstein W, Jewett S, Fox C F

出版信息

J Bacteriol. 1970 Nov;104(2):793-7. doi: 10.1128/jb.104.2.793-797.1970.

DOI:10.1128/jb.104.2.793-797.1970
PMID:4923073
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC285060/
Abstract

Mutants of Escherichia coli K-12 defective in enzyme I or Hpr, the two common components of the phosphoenolpyruvate-dependent phosphotransferase system, were isolated by a simple, direct method. The ptsI locus, the structural gene for enzyme I, and the ptsH locus, the site of mutations leading to loss of Hpr activity, are adjacent genes and could be part of a single operon. These two genes lie between the purC and supN markers in the order: strA... guaB-purC-ptsI-ptsH-supN-dsdA... his.

摘要

通过一种简单、直接的方法分离出了大肠杆菌K-12中磷酸烯醇丙酮酸依赖性磷酸转移酶系统的两个常见组分即酶I或Hpr有缺陷的突变体。酶I的结构基因ptsI位点和导致Hpr活性丧失的突变位点ptsH位点是相邻基因，可能是单个操纵子的一部分。这两个基因按以下顺序位于purC和supN标记之间：strA...guaB-purC-ptsI-ptsH-supN-dsdA...his。

相似文献

1
Isolation and mapping of phosphotransferase mutants in Escherichia coli.大肠杆菌中磷酸转移酶突变体的分离与定位
J Bacteriol. 1970 Nov;104(2):793-7. doi: 10.1128/jb.104.2.793-797.1970.
2
[Mapping of mutations within the genes coding for enzyme I and Hpr proteins of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli K-12. I. Mapping of the mutations within the ptsI gene].[大肠杆菌K-12磷酸烯醇丙酮酸依赖性磷酸转移酶系统中编码酶I和Hpr蛋白的基因突变定位。I. ptsI基因内突变的定位]
Genetika. 1981;17(10):1771-83.
3
[Mapping of the mutations within the genes coding for enzyme I and protein Hpr of the phosphoenolpyruvate-dependent phosphotransferase system in Escherichia coli K-12. II. Mapping of the mutations within gene ptsH].[大肠杆菌K-12中磷酸烯醇丙酮酸依赖性磷酸转移酶系统的酶I和蛋白Hpr编码基因内突变的定位。II. ptsH基因内突变的定位]
Genetika. 1983 Mar;19(3):397-405.
4
Deletion mapping of the genes coding for HPr and enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system in Salmonella typhimurium.鼠伤寒沙门氏菌中磷酸烯醇丙酮酸：糖磷酸转移酶系统的HPr和酶I编码基因的缺失定位
J Bacteriol. 1972 Oct;112(1):17-29. doi: 10.1128/jb.112.1.17-29.1972.
5
Sugar transport. Properties of mutant bacteria defective in proteins of the phosphoenolpyruvate: sugar phosphotransferase system.糖转运。磷酸烯醇丙酮酸：糖磷酸转移酶系统中蛋白质缺陷型突变细菌的特性。
J Biol Chem. 1976 Nov 10;251(21):6584-97.
6
Sugar transport by the bacterial phosphotransferase system. Molecular cloning and structural analysis of the Escherichia coli ptsH, ptsI, and crr genes.细菌磷酸转移酶系统介导的糖转运。大肠杆菌ptsH、ptsI和crr基因的分子克隆与结构分析。
J Biol Chem. 1987 Nov 25;262(33):16241-53.
7
Analysis of the ptsH-ptsI-crr region in Escherichia coli K-12: nucleotide sequence of the ptsH gene.大肠杆菌K-12中ptsH-ptsI-crr区域的分析：ptsH基因的核苷酸序列
Gene. 1985;35(1-2):199-207. doi: 10.1016/0378-1119(85)90172-6.
8
Sequence and expression of the genes for HPr (ptsH) and enzyme I (ptsI) of the phosphoenolpyruvate-dependent phosphotransferase transport system from Streptococcus mutans.变形链球菌磷酸烯醇丙酮酸依赖性磷酸转移酶转运系统中HPr（ptsH）和酶I（ptsI）基因的序列与表达
Infect Immun. 1994 Apr;62(4):1156-65. doi: 10.1128/iai.62.4.1156-1165.1994.
9
[Isolation and genetic study of Erwinia mutants devoid of common components of the phosphoenolpyruvate-dependent phosphotransferase system].[缺乏磷酸烯醇丙酮酸依赖性磷酸转移酶系统常见组分的欧文氏菌突变体的分离与遗传学研究]
Genetika. 2002 May;38(5):622-8.
10
The ptsH, ptsI, and crr genes of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system: a complex operon with several modes of transcription.大肠杆菌磷酸烯醇丙酮酸依赖性磷酸转移酶系统的ptsH、ptsI和crr基因：一个具有多种转录模式的复杂操纵子。
J Bacteriol. 1988 Sep;170(9):3827-37. doi: 10.1128/jb.170.9.3827-3837.1988.

引用本文的文献

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Identification of the structural gene of the PEP-phosphotransferase enzyme I in Bacillus subtilis Marburg.枯草芽孢杆菌马伯格株中磷酸烯醇式丙酮酸-磷酸转移酶I结构基因的鉴定
Mol Gen Genet. 1975;136(4):337-49. doi: 10.1007/BF00341718.
2
Linkage map of Escherichia coli K-12, edition 10: the traditional map.大肠杆菌K-12连锁图谱，第10版：传统图谱。
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
3
A second purine nucleoside phosphorylase in Escherichia coli K-12. I. Xanthosine phosphorylase regulatory mutants isolated as secondary-site revertants of a deoD mutant.大肠杆菌K-12中的第二种嘌呤核苷磷酸化酶。I. 作为deoD突变体的二次位点回复突变体分离得到的黄嘌呤核苷磷酸化酶调节突变体。
Mol Gen Genet. 1980;179(2):331-40. doi: 10.1007/BF00425461.
4
Regulation of genes coding for enzyme constituents of the bacterial phosphotransferase system.细菌磷酸转移酶系统酶成分编码基因的调控
J Bacteriol. 1980 Feb;141(2):658-63. doi: 10.1128/jb.141.2.658-663.1980.
5
Escherichia coli K-12 F-prime factors, old and new.大肠杆菌K-12 F-prime因子，新旧情况
Bacteriol Rev. 1972 Dec;36(4):587-607. doi: 10.1128/br.36.4.587-607.1972.
6
Linkage map of Escherichia coli strain K-12.大肠杆菌K-12菌株的连锁图谱。
Bacteriol Rev. 1972 Dec;36(4):504-24. doi: 10.1128/br.36.4.504-524.1972.
7
Deletion mapping of the genes coding for HPr and enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system in Salmonella typhimurium.鼠伤寒沙门氏菌中磷酸烯醇丙酮酸：糖磷酸转移酶系统的HPr和酶I编码基因的缺失定位
J Bacteriol. 1972 Oct;112(1):17-29. doi: 10.1128/jb.112.1.17-29.1972.
8
Mutations affecting the dissimilation of mannitol by Escherichia coli K-12.影响大肠杆菌K-12对甘露醇异化作用的突变
J Bacteriol. 1972 Aug;111(2):566-74. doi: 10.1128/jb.111.2.566-574.1972.
9
Carbohydrate transport and cyclic 3',5' adenosine monophosphate (cAMP) levels in a temperature sensitive phosphotransferase mutant of Escherichia coli.大肠杆菌温度敏感型磷酸转移酶突变体中的碳水化合物转运及环磷酸腺苷（cAMP）水平
Mol Gen Genet. 1974 Mar 6;129(1):1-10. doi: 10.1007/BF00269261.
10
Phenotypic suppression of phosphofructokinase mutations in Escherichia coli by constitutive expression of the glyoxylate shunt.通过乙醛酸循环的组成型表达对大肠杆菌中磷酸果糖激酶突变的表型抑制
J Bacteriol. 1974 Jun;118(3):1090-100. doi: 10.1128/jb.118.3.1090-1100.1974.

本文引用的文献

1
PHOSPHATE BOUND TO HISTIDINE IN A PROTEIN AS AN INTERMEDIATE IN A NOVEL PHOSPHO-TRANSFERASE SYSTEM.蛋白质中与组氨酸结合的磷酸盐作为新型磷转移酶系统中的中间体。
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Potassium-dependant mutants of Escherichia coli K-12.大肠杆菌K-12的钾依赖性突变体
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Carbohydrate metabolism in microorganisms.微生物中的碳水化合物代谢
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Mutagens which cause deletions in Escherichia coli.在大肠杆菌中引起缺失的诱变剂。
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Carbohydrate accumulation and metabolism in Escherichia coli: the close linkage and chromosomal location of ctr mutations.大肠杆菌中的碳水化合物积累与代谢：ctr突变的紧密连锁及染色体定位
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Genetic analysis of carbohydrate transport-deficient mutants of Salmonella typhimurium.鼠伤寒沙门氏菌碳水化合物转运缺陷型突变体的遗传分析。
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7
The role of a phosphoenolpyruvate-dependent kinase system in beta-glucoside catabolism in Escherichia coli.磷酸烯醇丙酮酸依赖性激酶系统在大肠杆菌β-葡萄糖苷分解代谢中的作用。
Proc Natl Acad Sci U S A. 1968 Mar;59(3):988-95. doi: 10.1073/pnas.59.3.988.
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Revised linkage map of Escherichia coli.大肠杆菌的修订连锁图谱。
Bacteriol Rev. 1967 Dec;31(4):332-53. doi: 10.1128/br.31.4.332-353.1967.
9
Mapping of the nadB locus adjacent to a previously undescribed purine locus in Escherichia coli K-12.大肠杆菌K-12中与一个先前未描述的嘌呤基因座相邻的nadB基因座的定位。
J Bacteriol. 1970 May;102(2):377-81. doi: 10.1128/jb.102.2.377-381.1970.