一种能修复大肠杆菌细胞分裂机制损伤的细胞组分的特性。
Properties of a cell fraction that repairs damage to the cell division mechanism of Escherichia coli.
作者信息
Fisher W D, Adler H I, Shull F W, Cohen A
出版信息
J Bacteriol. 1969 Feb;97(2):500-5. doi: 10.1128/jb.97.2.500-505.1969.
Abstract
A factor in bacterial cell extracts which induces cell division in filaments of Escherichia coli produced by irradiation was found to be associated with a heat-labile particulate fraction which sediments at about 100S. Frozen or lyophilized samples of the cell extracts were stable for considerable periods of time. Fractions purified by centrifugation contained 2% of the total protein of the extract but no measurable ribonucleic acid or deoxyribonucleic acid. The extracts were inactivated by incubation with phospholipase A, lipases, and detergents, but not by incubation with selected nucleases and proteases.
摘要
在细菌细胞提取物中发现一种可诱导经辐射产生的大肠杆菌丝状菌进行细胞分裂的因子，它与一种沉降系数约为100S的热不稳定颗粒部分相关。细胞提取物的冷冻或冻干样品在相当长的时间内保持稳定。通过离心纯化的部分含有提取物总蛋白的2%，但没有可测量的核糖核酸或脱氧核糖核酸。提取物经磷脂酶A、脂肪酶和去污剂处理后失活，但经选定的核酸酶和蛋白酶处理后未失活。
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