厌氧培养可增强大肠杆菌K-12的polA recB菌株的菌落形成。
Anaerobic incubation enhances the colony formation of a polA recB strain of Escherichia coli K-12.
作者信息
Morimyo M
出版信息
J Bacteriol. 1982 Oct;152(1):208-14. doi: 10.1128/jb.152.1.208-214.1982.
Abstract
Escherichia coli strain E247 (polA1 recB21) has reduced colony formation (even at the permissive temperature of 30 degrees C) because of a poor suppressor mutation (sup-126). The colony formation was enhanced in the absence of oxygen about 3-fold at 30 degrees C and 10(6)-fold at 43 degrees C, suggesting that a polA recB strain was inviable due to oxygen toxicity. Colony formation was also increased by incubation in an agar medium containing the reducing agent thioglycolate and incubation in the presence of chloroform-killed Saccharomyces cerevisiae pet+ cells, but not pet cells. Since the E247 strain viability was inversely dependent on the oxygen pressure and since the strain was more sensitive to superoxide radical than either the polA or the recB mutant, it seems likely that the polA and recB genes play a role in repairing DNA damage during respiration.
摘要
大肠杆菌菌株E247（polA1 recB21）由于抑制突变不佳（sup-126），其菌落形成减少（即使在30摄氏度的允许温度下）。在无氧条件下，30摄氏度时菌落形成增强约3倍，43摄氏度时增强10^6倍，这表明polA recB菌株因氧毒性而无法存活。在含有还原剂巯基乙酸盐的琼脂培养基中培养以及在氯仿杀死的酿酒酵母pet+细胞（而非pet细胞）存在的情况下培养，菌落形成也会增加。由于E247菌株的活力与氧压力呈负相关，且该菌株比polA或recB突变体对超氧自由基更敏感，因此polA和recB基因似乎在呼吸过程中修复DNA损伤方面发挥作用。
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