Duda J J, Slack J M
J Bacteriol. 1969 Feb;97(2):900-4. doi: 10.1128/jb.97.2.900-904.1969.
Spheroplasts of Clostridium botulinum 62A were prepared with the use of lysozyme. These spheroplasts were then exposed to ferritin-labeled type A antitoxin. Ultrathin sections of these specimens revealed the ferritin-labeled antibody symmetrically arranged around the outer spore coats but not within the spore cortex. The ferritin-labeled antibody was also observed in the bacterial cytoplasm. Here it was arranged in aggregates and strands, although it was not associated with any identifiable cell structure. Controls included sections of C. botulinum spheroplasts treated with a 1.5% solution of ferritin as well as spheroplasts of C. roseum and Bacillus subtilis treated with conjugated type A antitoxin or a 1.5% solution of ferritin. No intracellular or extracellular ferritin was demonstrable in these specimens.
肉毒梭菌62A的原生质球是用溶菌酶制备的。然后将这些原生质球暴露于铁蛋白标记的A型抗毒素中。这些标本的超薄切片显示，铁蛋白标记的抗体对称地排列在外孢子壁周围，而不在孢子皮层内。在细菌细胞质中也观察到了铁蛋白标记的抗体。在这里，它呈聚集状和条索状排列，尽管它与任何可识别的细胞结构都没有关联。对照包括用1.5%铁蛋白溶液处理的肉毒梭菌原生质球切片，以及用结合型A型抗毒素或1.5%铁蛋白溶液处理的玫瑰色梭菌和枯草芽孢杆菌的原生质球。在这些标本中未检测到细胞内或细胞外的铁蛋白。
