通过凝胶过滤从小鼠脾脏获得的弗氏病毒的免疫学特性。
Immunological properties of Friend virus from mouse spleen obtained by gel filtration.
作者信息
Chan S P, Chirigos M A, Hook W A
出版信息
Appl Microbiol. 1969 Jan;17(1):157-63. doi: 10.1128/am.17.1.157-163.1969.
Abstract
Gel filtration, with Sephadex G-200, was utilized to obtain Friend virus (FV) from infected spleen homogenate. Highly infectious and immunologically active material was eluted as a large initial protein peak, whereas hemoglobin and other noninfectious materials were found in two subsequent peaks. Fractions that made up the first peak were reactive in serological tests with antiserum towards FV. Formalinized fractions from the first peak, when combined with Freund's complete adjuvant, were able to protect mice against subsequent FV challenge. Further purification of antigen was carried out by sucrose gradient ultracentrifugation.
摘要
使用葡聚糖凝胶G - 200进行凝胶过滤，从感染的脾脏匀浆中获取弗氏病毒（FV）。高传染性和免疫活性物质作为一个大的初始蛋白峰被洗脱出来，而血红蛋白和其他非传染性物质则出现在随后的两个峰中。构成第一个峰的组分在血清学试验中与抗FV血清发生反应。来自第一个峰的经福尔马林处理的组分与弗氏完全佐剂混合后，能够保护小鼠免受随后的FV攻击。通过蔗糖梯度超速离心对抗原进行进一步纯化。
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Appl Microbiol. 1969 Jan;17(1):157-63. doi: 10.1128/am.17.1.157-163.1969.
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本文引用的文献
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