Lerke P, Farber L
Appl Microbiol. 1969 Feb;17(2):197-201. doi: 10.1128/am.17.2.197-201.1969.
Comparison of various indices of deterioration of refrigerated fish fillets showed that the direct bacterial count can be used to predict the storage life of the foodstuff. For direct counts, a thin film made from fillet surface material was spread on a microscope slide, stained, and read. Initial counts were found to correlate well with keeping quality; a period of freshness of 24 or 48 hr at 5 C could be reliably predicted. Preliminary data indicated that hypoxanthine estimation could probably also be used for the prediction of shelf life but that the relative complexity of the procedure detracted from its usefulness.
对冷藏鱼片各种变质指标的比较表明，直接细菌计数可用于预测该食品的储存期。进行直接计数时，将由鱼片表面材料制成的薄膜铺展在显微镜载玻片上，染色后进行读数。发现初始计数与保鲜质量密切相关；在5℃下24或48小时的新鲜期可以得到可靠预测。初步数据表明，次黄嘌呤估计法可能也可用于预测货架期，但该方法相对复杂，影响了其实用性。
