Chai T, Chen C, Rosen A, Levin R E
Appl Microbiol. 1968 Nov;16(11):1738-41. doi: 10.1128/am.16.11.1738-1741.1968.
Pseudomonas putrefaciens has been found to constitute one of the major species of spoilage bacteria on haddock fillets. The initial population of this organism on fillets of high bacterial quality is uniformly below 4% and most frequently no greater than 1%. During refrigerated storage, the organism increases at a more rapid rate than the total psychrophilic population, comprising 50 to 90% of the total population when the total count exceeds 10(6)/g of tissue. Fluorescent pseudomonads were shown to constitute a second group of predominant pseudomonads constituting up to 19.3% of the total population after 8 days of refrigerated storage. Of a total of 45 fluorescent pseudomonads isolated from haddock fillets, 14 (31.1%) were found to be potent fish spoilers. The use of a soft-agar-gelatin plating technique showed a parallel increase of proteolytic organisms with total count indicating that proteolytic organisms other than P. putrefaciens and fluorescent pseudomonads increase at a slower rate than these two groups.
已发现腐败希瓦氏菌是黑线鳕鱼片上主要的腐败细菌种类之一。在细菌质量较高的鱼片上，这种微生物的初始数量普遍低于4%，且多数情况下不超过1%。在冷藏储存期间，该微生物的增长速度比嗜冷菌总数更快，当细菌总数超过10⁶/g组织时，它占总菌数的50%至90%。荧光假单胞菌是另一组主要的假单胞菌，冷藏储存8天后，其数量占总菌数的比例高达19.3%。从黑线鳕鱼片分离出的45株荧光假单胞菌中，有14株（31.1%）被发现是强效的鱼类腐败菌。使用软琼脂 - 明胶平板培养技术显示，蛋白水解菌数量随总菌数平行增加，这表明除腐败希瓦氏菌和荧光假单胞菌外的蛋白水解菌增长速度比这两组慢。
