mRNA密码子的易位。II. 抗易位酶抗体的特性。
Translocation of mRNA codons. II. Properties of an anti-translocase antibody.
作者信息
Leder P, Skogerson L E, Roufa D J
出版信息
Proc Natl Acad Sci U S A. 1969 Mar;62(3):928-33. doi: 10.1073/pnas.62.3.928.
A purified preparation of translocase, one of several enzymes required for protein biosynthesis, has been used to prepare a specific anti-translocase antibody. This antibody provides an extremely useful tool not only for detecting the enzyme independent of its activity, but for inhibiting the translocase-mediated reaction and, thus, protein biosynthesis. Though the antibody very rapidly inhibits elongation of the peptide chain, it fails to effect initiation, binding, or peptide bond formation, which strongly suggests that translocase is not a direct participant in these reactions. It also arrests translation of the defined tricodon AUG(U(6)) at the second codon, permitting formation only of the dipeptide, fMet-Phe, rather than the tripeptide, fMetPhe-(Phe)(2), which is formed in the presence of control serum. We have shown previously that the third codon becomes available only in the presence of translocase, thereby defining the site of action of the antibody. The antibody also has been used to demonstrate that translocase is antigenically distinct from the other proteins required for initiation and protein synthesis in E. coli.
转位酶是蛋白质生物合成所需的几种酶之一，一种纯化的转位酶制剂已被用于制备特异性抗转位酶抗体。这种抗体不仅为独立于其活性检测该酶提供了极为有用的工具，而且用于抑制转位酶介导的反应，进而抑制蛋白质生物合成。尽管该抗体能非常迅速地抑制肽链的延伸，但它对起始、结合或肽键形成没有影响，这有力地表明转位酶不是这些反应的直接参与者。它还会使特定的三联密码子AUG（U(6)）在第二个密码子处停止翻译，仅允许形成二肽fMet-Phe，而不是在对照血清存在时形成的三肽fMetPhe-(Phe)(2)。我们之前已经表明，只有在转位酶存在的情况下第三个密码子才可用，从而确定了抗体的作用位点。该抗体还被用于证明转位酶在抗原性上与大肠杆菌中起始和蛋白质合成所需的其他蛋白质不同。
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