通过酶联免疫吸附测定法检测人血清中抗小肠结肠炎耶尔森菌脂多糖的抗体
Demonstration of antibodies against Yersinia enterocolitica lipopolysaccharide in human sera by enzyme-linked immunosorbent assay.
作者信息
Gripenberg M, Nissinen A, Väisänen E, Linder E
出版信息
J Clin Microbiol. 1979 Sep;10(3):279-84. doi: 10.1128/jcm.10.3.279-284.1979.
Abstract
Antibodies against Yersinia enterocolitica serotype O:3 lipopolysaccharide present in sera from patients with Yersinia infection were studied by using an enzyme-linked immunosorbent assay. Of the sera with significant bacterial agglutination titers against Y. enterocolitica type O:3, 86% contained anti-lipopolysaccharide antibodies of the immunoglobulin G class. With the sera of some patients, we demonstrated increasing anti-lipopolysaccharide antibody levels of immunoglobulin G class in spite of decreasing bacterial agglutination titers. The assay was specific for lipopolysaccharide from Y. enterocolitica type O:3, and in inhibition experiments lipopolysaccharide could be detected in amounts of greater than or equal to 0.5 micrograms/ml.
摘要
采用酶联免疫吸附测定法，对耶尔森氏菌感染患者血清中抗小肠结肠炎耶尔森氏菌O:3血清型脂多糖的抗体进行了研究。在对小肠结肠炎耶尔森氏菌O:3具有显著细菌凝集效价的血清中，86%含有免疫球蛋白G类抗脂多糖抗体。对于一些患者的血清，我们发现尽管细菌凝集效价降低，但免疫球蛋白G类抗脂多糖抗体水平仍在升高。该测定法对小肠结肠炎耶尔森氏菌O:3型脂多糖具有特异性，在抑制实验中，可检测到含量大于或等于0.5微克/毫升的脂多糖。
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