钠刺激下大肠杆菌中谷氨酸的转运
Sodium-stimulated transport of glutamate in Escherichia coli.
作者信息
Frank L, Hopkins I
出版信息
J Bacteriol. 1969 Oct;100(1):329-36. doi: 10.1128/jb.100.1.329-336.1969.
Abstract
Wild-type Escherichia coli B grew poorly on glutamate as the sole carbon source, except at very high concentrations of the amino acid. The addition of sodium ion markedly stimulated the growth. It had the same effect in a mutant of E. coli B selected for the ability to grow at low glutamate concentrations. Sodium ion also potentiated growth inhibition by analogues of glutamate. The uptake of glutamate by nongrowing cells of the mutant was markedly stimulated by sodium ion in the presence of an energy source, chloramphenicol, and arsenite, which retarded glutamate degradation.
摘要
野生型大肠杆菌B在以谷氨酸作为唯一碳源时生长不佳，除非氨基酸浓度非常高。钠离子的添加显著刺激了生长。在为能够在低谷氨酸浓度下生长而选择的大肠杆菌B突变体中也有相同的效果。钠离子还增强了谷氨酸类似物对生长的抑制作用。在存在能量源、氯霉素和亚砷酸盐（其会阻碍谷氨酸降解）的情况下，钠离子显著刺激了突变体非生长细胞对谷氨酸的摄取。
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