用于检测非洲猪瘟抗原和抗体的固相放射免疫测定技术。
Solid-phase radioimmunoassay techniques for the detection of African swine fever antigen and antibody.
作者信息
Crowther J R, Wardley R C, Wilkinson P J
出版信息
J Hyg (Lond). 1979 Oct;83(2):353-61. doi: 10.1017/s0022172400026140.
Abstract
A solid phase radioimmunoassay (RIA) has been successfully developed to measure both African swine fever virus (ASFV) antigen and antibody. Studies show that the assay is reproducible and will detect limiting antigen concentrations equivalent to 50--500 HAD50/ml. Both direct and indirect antibody RIA have been developed and have proved to be approximately 100 times fore sensitive than the complement fixation test at present available and 1000 times more sensitive than the immuno-electro-osmophoresis test for the detection of ASFV antibody.
摘要
一种固相放射免疫测定法（RIA）已成功开发出来，用于检测非洲猪瘟病毒（ASFV）抗原和抗体。研究表明，该测定法具有可重复性，能检测低至50--500个半数血细胞吸附剂量（HAD50）/毫升的抗原浓度。直接和间接抗体RIA均已开发出来，事实证明，在检测ASFV抗体方面，它们的灵敏度比目前可用的补体结合试验高约100倍，比免疫电渗电泳试验高1000倍。
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