• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

用于检测非洲猪瘟抗原和抗体的固相放射免疫测定技术。

Solid-phase radioimmunoassay techniques for the detection of African swine fever antigen and antibody.

作者信息

Crowther J R, Wardley R C, Wilkinson P J

出版信息

J Hyg (Lond). 1979 Oct;83(2):353-61. doi: 10.1017/s0022172400026140.

DOI:10.1017/s0022172400026140
PMID:489964
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2129899/
Abstract

A solid phase radioimmunoassay (RIA) has been successfully developed to measure both African swine fever virus (ASFV) antigen and antibody. Studies show that the assay is reproducible and will detect limiting antigen concentrations equivalent to 50--500 HAD50/ml. Both direct and indirect antibody RIA have been developed and have proved to be approximately 100 times fore sensitive than the complement fixation test at present available and 1000 times more sensitive than the immuno-electro-osmophoresis test for the detection of ASFV antibody.

摘要

一种固相放射免疫测定法(RIA)已成功开发出来,用于检测非洲猪瘟病毒(ASFV)抗原和抗体。研究表明,该测定法具有可重复性,能检测低至50--500个半数血细胞吸附剂量(HAD50)/毫升的抗原浓度。直接和间接抗体RIA均已开发出来,事实证明,在检测ASFV抗体方面,它们的灵敏度比目前可用的补体结合试验高约100倍,比免疫电渗电泳试验高1000倍。

相似文献

1
Solid-phase radioimmunoassay techniques for the detection of African swine fever antigen and antibody.用于检测非洲猪瘟抗原和抗体的固相放射免疫测定技术。
J Hyg (Lond). 1979 Oct;83(2):353-61. doi: 10.1017/s0022172400026140.
2
A solid-phase enzyme linked immunosorbent assay for the detection of African swine fever virus antigen and antibody.一种用于检测非洲猪瘟病毒抗原和抗体的固相酶联免疫吸附测定法。
J Hyg (Lond). 1979 Oct;83(2):363-9. doi: 10.1017/s0022172400026152.
3
A simplified method for detection of African swine fever antibodies by immunoelectroosmophoresis.一种通过免疫电渗法检测非洲猪瘟抗体的简化方法。
Bull Pan Am Health Organ. 1980;14(3):229-37.
4
Evaluation of sensitivity of different antigen and DNA-hybridization methods in African swine fever virus detection.不同抗原和DNA杂交方法在非洲猪瘟病毒检测中的敏感性评估。
J Virol Methods. 1990 Apr;28(1):67-77. doi: 10.1016/0166-0934(90)90088-w.
5
Monoclonal antibodies against African swine fever viral antigens.
Virology. 1985 Apr 30;142(2):416-20. doi: 10.1016/0042-6822(85)90350-2.
6
African swine fever antibody detection in warthogs.疣猪体内非洲猪瘟抗体检测
Vet Rec. 1979 Jul 21;105(3):61. doi: 10.1136/vr.105.3.61.
7
Detection of antibodies against African swine fever virus using infected monolayers and monoclonal antibodies.使用感染的单层细胞和单克隆抗体检测抗非洲猪瘟病毒抗体。
Vet Rec. 1988 May 28;122(22):536-9. doi: 10.1136/vr.122.22.536.
8
Detection of African swine fever virus antibodies by immunoblotting assay.通过免疫印迹法检测非洲猪瘟病毒抗体。
Can J Vet Res. 1989 Jan;53(1):105-7.
9
The immunological response of pigs and guinea pigs to antigens of African swine fever virus.猪和豚鼠对非洲猪瘟病毒抗原的免疫反应。
Arch Virol. 1982;74(2-3):91-100. doi: 10.1007/BF01314703.
10
Characterization of African swine fever virus antigenic proteins by immunoprecipitation.通过免疫沉淀法对非洲猪瘟病毒抗原蛋白进行表征
Arch Virol. 1984;80(4):265-74. doi: 10.1007/BF01311218.

引用本文的文献

1
Prevalence of African swine fever virus in apparently healthy domestic pigs in Uganda.乌干达貌似健康的家猪中非洲猪瘟病毒的流行情况。
BMC Vet Res. 2013 Dec 26;9:263. doi: 10.1186/1746-6148-9-263.
2
The role of nuclear technologies in the diagnosis and control of livestock diseases--a review.核技术在牲畜疾病诊断与防控中的作用——综述
Trop Anim Health Prod. 2012 Oct;44(7):1341-66. doi: 10.1007/s11250-012-0077-5.
3
African Swine Fever virus. Brief review.非洲猪瘟病毒。简要综述。
Arch Virol. 1983;76(2):73-90. doi: 10.1007/BF01311692.
4
Effect of African swine fever on lymphocyte mitogenesis.非洲猪瘟对淋巴细胞有丝分裂的影响。
Immunology. 1982 May;46(1):215-20.
5
A solid-phase enzyme linked immunosorbent assay for the detection of African swine fever virus antigen and antibody.一种用于检测非洲猪瘟病毒抗原和抗体的固相酶联免疫吸附测定法。
J Hyg (Lond). 1979 Oct;83(2):363-9. doi: 10.1017/s0022172400026152.

本文引用的文献

1
Hemadsorption and cytopathic effect produced by African Swine Fever virus in swine bone marrow and buffy coat cultures.非洲猪瘟病毒在猪骨髓和血沉棕黄层培养物中产生的血细胞吸附和细胞病变效应。
Am J Vet Res. 1960 Jan;21:104-8.
2
PROPAGATION AND MODIFICATION OF AFRICAN SWINE FEVER VIRUS IN CELL CULTURES.非洲猪瘟病毒在细胞培养中的增殖与变异
Am J Vet Res. 1965 Jan;26:141-6.
3
Procomplementary activity of swine serum and its elimination with periodate treatment.
Jpn J Med Sci Biol. 1962 Aug;15:165-73. doi: 10.7883/yoken1952.15.165.
4
The morphological characteristics of African swine fever virus and its resemblance to tipula iridescent virus.非洲猪瘟病毒的形态特征及其与大蚊虹彩病毒的相似性。
Arch Gesamte Virusforsch. 1967;20(3):392-6. doi: 10.1007/BF01241958.
5
Fluorescent antibody studies on African swine fever virus.非洲猪瘟病毒的荧光抗体研究
Am J Vet Res. 1966 Mar;27(117):477-84.
6
Comparison of direct and indirect solid-phase microradioimmunoassays for the detection of viral antigens and antiviral antibody.用于检测病毒抗原和抗病毒抗体的直接和间接固相微放射免疫测定法的比较。
Appl Microbiol. 1973 Apr;25(4):567-73. doi: 10.1128/am.25.4.567-573.1973.
7
African swine fever: comparison of four serotests on porcine serums in Spain.非洲猪瘟:西班牙猪血清四项血清学检测方法的比较
Am J Vet Res. 1974 Jun;35(6):787-90.
8
African swine fever: application of immunoelectroosmophoresis for the detection of antibody.非洲猪瘟:免疫电渗法在抗体检测中的应用
Can J Comp Med. 1972 Jul;36(3):309-16.
9
Successful prophylaxis against rabies in mice and Rhesus monkeys: the interferon system and vaccine.
J Infect Dis. 1977 Aug;136(2):286-91. doi: 10.1093/infdis/136.2.286.