College of Veterinary Medicine, Animal resources and Biosecurity, Makerere University, P,O,BOX 7062, Kampala, Uganda.
BMC Vet Res. 2013 Dec 26;9:263. doi: 10.1186/1746-6148-9-263.
African swine fever (ASF) is a contagious viral disease which can cause up to 100% mortality among domestic pigs leading to serious socio-economic impact on people's livelihoods. ASF is endemic in Uganda and there is paucity of information on the epidemiology of the disease. The major aim of this study was to determine the seroprevalence and prevalence of African swine fever virus (ASFV) in apparently healthy slaughter pigs at Wambizi slaughterhouse in Kampala city, Uganda. We also estimated the presence of ASFV antibodies and circulating viral antigens in pigs from selected districts of Uganda during targeted surveillance. We analysed 540 and 181 blood samples collected from slaughter pigs and pigs from targeted surveillance districts respectively.
The prevalence of ASFV in slaughter pigs was 52.96% (95% CI, 48.75-57.14) and 11.5% (95% CI, 9.06-14.45) by ELISA and PCR respectively. In surveillance districts, the proportion of ASFV positive pigs was 53.59% (95% CI, 46.33-60.71) and 0.55% (95% CI, 0.1-3.06) by ELISA and PCR respectively.
The study has found out a high seroprevalence of ASFV antibodies in apparently healthy slaughter pigs and also a high proportion of ASFV antibody seropositive pigs in surveyed districts in Uganda indicating exposure to ASFV. However, there was a lower prevalence of ASFV infection implying that there could be low virulent strains of ASFV circulating in domestic pigs in Uganda which requires further investigation.
非洲猪瘟（ASF）是一种传染性病毒病，可导致家猪 100%死亡，对人们的生计造成严重的社会经济影响。ASF 在乌干达流行，关于该病的流行病学信息很少。本研究的主要目的是确定乌干达坎帕拉市 Wambizi 屠宰场的健康屠宰猪的非洲猪瘟病毒（ASFV）血清流行率和流行率。我们还估计了在有针对性的监测中，乌干达选定地区的猪中 ASFV 抗体和循环病毒抗原的存在。我们分析了分别从屠宰猪和有针对性监测地区收集的 540 份和 181 份血液样本。
屠宰猪中 ASFV 的流行率分别通过 ELISA 和 PCR 检测为 52.96%（95%CI，48.75-57.14）和 11.5%（95%CI，9.06-14.45）。在监测地区，ASFV 阳性猪的比例分别通过 ELISA 和 PCR 检测为 53.59%（95%CI，46.33-60.71）和 0.55%（95%CI，0.1-3.06）。
该研究发现，健康屠宰猪的 ASFV 抗体血清流行率较高，乌干达调查地区的 ASFV 抗体血清阳性猪比例也较高，表明接触过 ASFV。然而，ASFV 感染的流行率较低，这意味着乌干达的家猪中可能存在低毒力的 ASFV 毒株，这需要进一步调查。
