大肠杆菌中诱导乳糖转运所需的脂质。
A lipid requirement for induction of lactose transport in Escherichia coli.
作者信息
Fox C F
出版信息
Proc Natl Acad Sci U S A. 1969 Jul;63(3):850-5. doi: 10.1073/pnas.63.3.850.
Abstract
The rate of derepressed synthesis of a membrane protein required for lactose transport (M protein) by Escherichia coli is increased in response to increased gene dosage to the same extent as the rates of synthesis of beta-galactosidase and galactoside acetylase. However, elevated gene dosage does not increase beta-galactoside transport to the same extent that it increases synthesis of M protein and of the soluble proteins of the lac operon. Though the factor or factors other than M protein which limit induction of the transport system at high levels of lac operon expression have not been identified, studies with Escherichia coli mutants blocked in the synthesis of unsaturated fatty acids indicate that unsaturated fatty acids must be supplied during the course of induction of the lac operon to permit synthesis of a functional lactose transport system, but not of beta-galactosidase or galactoside acetylase.
摘要
大肠杆菌乳糖转运所需膜蛋白（M蛋白）的去阻遏合成速率，会随着基因剂量增加而相应提高，其提高程度与β-半乳糖苷酶和半乳糖苷乙酰化酶的合成速率相同。然而，基因剂量升高时，β-半乳糖苷转运的增加幅度，不及M蛋白和乳糖操纵子可溶性蛋白合成的增加幅度。虽然在乳糖操纵子高表达水平下限制转运系统诱导的一个或多个因素尚未明确，但对不饱和脂肪酸合成受阻的大肠杆菌突变体的研究表明，在乳糖操纵子诱导过程中必须提供不饱和脂肪酸，才能合成功能性乳糖转运系统，但β-半乳糖苷酶或半乳糖苷乙酰化酶的合成则无需不饱和脂肪酸。
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Specific labeling and partial purification of the M protein, a component of the beta-galactoside transport system of Escherichia coli.大肠杆菌β-半乳糖苷转运系统组分M蛋白的特异性标记与部分纯化
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