Mariotti S, Oger J J, Fragu P, Antel J P, Kuo H H, DeGroot L J
J Immunol Methods. 1980;35(3-4):189-99. doi: 10.1016/0022-1759(80)90246-x.
We describe a simple solid-phase radioimmunoassay (RIA) to detect IgG based on competitive binding between radiolabeled and unlabeled IgG for anti-IgG antibody physically adsorbed to the wells of polyvinyl microtiter plates. The assay is sensitive (1 ng), rapid, and is particularly suited for studies of in vitro IgG secretion by human peripheral blood lymphocytes, since such studies require large numbers of cultures. Conditions which permit measurement, by means of this assay, of helper and suppressor T cell effects on IgG production by human B cells in culture are described.
我们描述了一种简单的固相放射免疫测定法（RIA），用于检测IgG。该方法基于放射性标记和未标记的IgG之间与物理吸附在聚乙烯微量滴定板孔中的抗IgG抗体的竞争性结合。该测定法灵敏（1纳克）、快速，特别适用于研究人外周血淋巴细胞的体外IgG分泌，因为此类研究需要大量培养物。本文还描述了通过该测定法测量辅助性T细胞和抑制性T细胞对培养中的人B细胞产生IgG的影响的条件。
