噬菌体T4 DNA依赖性溶菌酶的体外合成
Bacteriophage T4 DNA-dependent in vitro synthesis of lysozyme.
作者信息
Schweiger M, Gold L M
出版信息
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1351-8. doi: 10.1073/pnas.63.4.1351.
Abstract
A cell-free system derived from uninfected Escherichia coli previously was shown to synthesize beta-glucosyl transferase in response to T4 DNA. This same in vitro system, when incubated at slightly higher magnesium concentrations, also synthesized enzymatically active lysozyme. The lysozyme activity that appeared was judged to be T4-specific since antibodies prepared against authentic T4-lysozyme inactivated the in vitro synthesized enzyme. DNA from a T4 mutant carrying a deletion in the lysozyme gene stimulated amino acid incorporation to the same extent as wild-type T4 DNA but was inactive in directing the synthesis of lysozyme. Various inhibitors of RNA and protein synthesis inhibited the in vitro synthesis of lysozyme.
摘要
先前已证明，从未感染的大肠杆菌中提取的无细胞系统可响应T4 DNA合成β-葡萄糖基转移酶。当在略高的镁浓度下孵育时，这个相同的体外系统也会合成具有酶活性的溶菌酶。出现的溶菌酶活性被判定为T4特异性的，因为针对正宗T4溶菌酶制备的抗体使体外合成的酶失活。携带溶菌酶基因缺失的T4突变体的DNA刺激氨基酸掺入的程度与野生型T4 DNA相同，但在指导溶菌酶合成方面无活性。RNA和蛋白质合成的各种抑制剂抑制了溶菌酶的体外合成。
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