由T偶数噬菌体DNA体外指导合成噬菌体特异性α-和β-葡萄糖基转移酶
Synthesis of phage-specific alpha- and beta-glucosyl transferases directed by T-even DNA in vitro.
作者信息
Gold L M, Schweiger M
出版信息
Proc Natl Acad Sci U S A. 1969 Mar;62(3):892-8. doi: 10.1073/pnas.62.3.892.
Abstract
-Bacteriophage T4 DNA, when added to a ribonucleic acid- and protein-synthesizing system from uninfected Escherichia coli, directed the in vitro synthesis of virus-specific glucosyl transferases. The T4-specific alpha- and beta-glucosyl transferases are synthesized in vivo early after infection, and function to glucosylate the hydroxymethylcytosine residues of phage DNA. The in vitro glucosyl transferase synthesis was dependent upon transcription of T4 DNA, as well as upon protein synthesis. DNA from T4 mutants unable to induce glucosyl transerases failed to induce enzyme formation in vitro, although protein synthesis was unimpaired.
摘要
将噬菌体T4 DNA添加到未感染的大肠杆菌的核糖核酸和蛋白质合成系统中时，可指导病毒特异性葡糖基转移酶的体外合成。T4特异性α-和β-葡糖基转移酶在感染后早期在体内合成，并作用于使噬菌体DNA的羟甲基胞嘧啶残基葡糖基化。体外葡糖基转移酶的合成依赖于T4 DNA的转录以及蛋白质合成。来自无法诱导葡糖基转移酶的T4突变体的DNA在体外无法诱导酶的形成，尽管蛋白质合成未受影响。
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