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Synthesis of phage-specific alpha- and beta-glucosyl transferases directed by T-even DNA in vitro.由T偶数噬菌体DNA体外指导合成噬菌体特异性α-和β-葡萄糖基转移酶
Proc Natl Acad Sci U S A. 1969 Mar;62(3):892-8. doi: 10.1073/pnas.62.3.892.
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本文引用的文献

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Glucosylation of deoxyribonucleic acid. III. alpha- and beta-Glucosyl transferases from T4-infected Escherichia coli.脱氧核糖核酸的糖基化作用。III. 来自T4噬菌体感染的大肠杆菌的α-和β-葡萄糖基转移酶
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The role of deoxyribonucleic acid in ribonucleic acid synthesis. III. The inhibition of the enzymatic synthesis of ribonucleic acid and deoxyribonucleic acid by actinomycin D and proflavin.脱氧核糖核酸在核糖核酸合成中的作用。III. 放线菌素D和原黄素对核糖核酸和脱氧核糖核酸酶促合成的抑制作用。
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On the structure of the glucosylated hydroxymethylcytosine nucleotides of coliphages T2, T4, and T6.关于大肠杆菌噬菌体T2、T4和T6的糖基化羟甲基胞嘧啶核苷酸的结构
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Glucosylation of deoxyribonucleic acid by enzymes from bacteriophage-infected Escherichia coli.噬菌体感染的大肠杆菌中酶对脱氧核糖核酸的糖基化作用。
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In vitro synthesis of bacteriophage lysozyme.噬菌体溶菌酶的体外合成
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Isolation and preliminary characterization of T4 mutants with nonglucosylated DNA.具有非糖基化DNA的T4突变体的分离与初步表征
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A factor from E. coli required for the translation of natural messenger RNA.一种来自大肠杆菌的、天然信使核糖核酸翻译所必需的因子。
Biochem Biophys Res Commun. 1966 Oct 5;25(1):124-32. doi: 10.1016/0006-291x(66)90649-8.
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Selective synthesis of T-even bacteriophage early messenger in vitro.T偶数噬菌体早期信使核糖核酸的体外选择性合成
J Mol Biol. 1966 Aug;19(2):541-7. doi: 10.1016/s0022-2836(66)80021-9.
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Nucleotide diphosphate hexose pyrophosphatases.核苷酸二磷酸己糖焦磷酸酶
Biochem Biophys Res Commun. 1966 Mar 8;22(5):524-31. doi: 10.1016/0006-291x(66)90306-8.
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[Studies on the biosynthesis of proteins. VII. Activity of various desoxyribonucleic acids and ribonuclease inhibitors from rabbit reticulocytes in a cell-free protein synthesis system in Escherichia coli. DNA-dependent in vitro synthesis of "early proteins" of E. coliphages T4].[蛋白质生物合成的研究。VII. 兔网织红细胞中各种脱氧核糖核酸和核糖核酸酶抑制剂在大肠杆菌无细胞蛋白质合成系统中的活性。大肠杆菌噬菌体T4“早期蛋白质”的DNA依赖性体外合成]
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由T偶数噬菌体DNA体外指导合成噬菌体特异性α-和β-葡萄糖基转移酶

Synthesis of phage-specific alpha- and beta-glucosyl transferases directed by T-even DNA in vitro.

作者信息

Gold L M, Schweiger M

出版信息

Proc Natl Acad Sci U S A. 1969 Mar;62(3):892-8. doi: 10.1073/pnas.62.3.892.

DOI:10.1073/pnas.62.3.892
PMID:4895217
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC223682/
Abstract

-Bacteriophage T4 DNA, when added to a ribonucleic acid- and protein-synthesizing system from uninfected Escherichia coli, directed the in vitro synthesis of virus-specific glucosyl transferases. The T4-specific alpha- and beta-glucosyl transferases are synthesized in vivo early after infection, and function to glucosylate the hydroxymethylcytosine residues of phage DNA. The in vitro glucosyl transferase synthesis was dependent upon transcription of T4 DNA, as well as upon protein synthesis. DNA from T4 mutants unable to induce glucosyl transerases failed to induce enzyme formation in vitro, although protein synthesis was unimpaired.

摘要

将噬菌体T4 DNA添加到未感染的大肠杆菌的核糖核酸和蛋白质合成系统中时，可指导病毒特异性葡糖基转移酶的体外合成。T4特异性α-和β-葡糖基转移酶在感染后早期在体内合成，并作用于使噬菌体DNA的羟甲基胞嘧啶残基葡糖基化。体外葡糖基转移酶的合成依赖于T4 DNA的转录以及蛋白质合成。来自无法诱导葡糖基转移酶的T4突变体的DNA在体外无法诱导酶的形成，尽管蛋白质合成未受影响。