大肠杆菌DNA复制的负调控机制。
The negative control mechanism for E. coli DNA replication.
作者信息
Rosenberg B H, Cavalieri L F, Ungers G
出版信息
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1410-7. doi: 10.1073/pnas.63.4.1410.
Abstract
Evidence is presented to show that the initiation of DNA replication in E. coli 555-7 requires synthesis of a protein whose production is correlated with total protein synthesis. Once replication is initiated, however, reinitiation will occur if all further protein synthesis is prevented; a small amount of protein synthesis is sufficient to prevent this unregulated reinitiation. This shows that the initiation of DNA replication is under negative control. A mechanism for the control of DNA replication is proposed; in this mechanism a replication repressor is synthesized periodically, while an antirepressor protein is synthesized continuously. Derepression of initiation results after sufficient accumulation of the antirepressor protein, and repression is re-established by repressor synthesis after the initiation of replication.
摘要
有证据表明，大肠杆菌555 - 7中DNA复制的起始需要一种蛋白质的合成，该蛋白质的产生与总蛋白质合成相关。然而，一旦复制开始，如果阻止所有进一步的蛋白质合成，重新起始仍会发生；少量的蛋白质合成就足以阻止这种不受调控的重新起始。这表明DNA复制的起始受到负调控。本文提出了一种DNA复制的控制机制；在该机制中，复制阻遏物周期性合成，而抗阻遏蛋白持续合成。在抗阻遏蛋白充分积累后，起始去阻遏，复制开始后阻遏蛋白的合成重新建立阻遏状态。
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