大肠杆菌一种需要半胱氨酸的“松弛型”突变体中的缺硫转移核糖核酸
Sulfur-deficient transfer ribonucleic acid in a cysteine-requiring, "relaxed" mutant of Escherichia coli.
作者信息
Harris C L, Titchener E B, Cline A L
出版信息
J Bacteriol. 1969 Dec;100(3):1322-7. doi: 10.1128/jb.100.3.1322-1327.1969.
Abstract
A cysteine-requiring mutant of the parent strain Escherichia coli Hfr Cavalli (RC(rel), Met(-), lambda) has been isolated. The mutant was selected by using replica plating after mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine. The mutation appears to be in the gene for sulfite reductase, since the mutant could utilize sulfide but not sulfite as a sulfur source. The mutant was found to be RC(rel) with respect to both methionine and cysteine. During cysteine starvation, transfer ribonucleic acid (tRNA) deficient in 4-thiouracil was produced, and in vivo studies indicate that this tRNA can accept sulfur groups to a greater extent than normal tRNA. Further, there were differences both in the rate and extent of amino acid acceptance between normal and sulfur-deficient tRNA. This suggests that thionucleotides are involved in at least one of the biological functions of the tRNA molecule.
摘要
已分离出亲本菌株大肠杆菌Hfr卡瓦利（RC(rel)，Met(-)，λ）的一个半胱氨酸需求型突变体。该突变体是在用N-甲基-N'-硝基-N-亚硝基胍诱变后通过影印平板法筛选得到的。该突变似乎发生在亚硫酸盐还原酶基因中，因为该突变体可以利用硫化物但不能利用亚硫酸盐作为硫源。发现该突变体在甲硫氨酸和半胱氨酸方面均为RC(rel)。在半胱氨酸饥饿期间，产生了缺乏4-硫尿嘧啶的转移核糖核酸（tRNA），体内研究表明，这种tRNA比正常tRNA能更大程度地接受硫基团。此外，正常tRNA和缺硫tRNA在氨基酸接受的速率和程度上均存在差异。这表明硫代核苷酸至少参与了tRNA分子的一种生物学功能。
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本文引用的文献
1
Thiobases in Escherchia coli Transfer RNA: 2-Thiocytosine and 5-Methylaminomethyl-2-thiouracil.大肠杆菌转移 RNA 中的硫碱基：2-硫胞嘧啶和 5-甲氨甲基-2-硫尿嘧啶。
Science. 1968 Sep 13;161(3846):1146-7. doi: 10.1126/science.161.3846.1146.
2
Replica plating and indirect selection of bacterial mutants.细菌突变体的影印培养和间接筛选
J Bacteriol. 1952 Mar;63(3):399-406. doi: 10.1128/jb.63.3.399-406.1952.
3
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
4
THE NATURE OF THE RNA SYNTHESIZED DURING CONDITIONS OF UNBALANCED GROWTH IN E. COLI K12W-6.大肠杆菌K12W-6不平衡生长条件下合成的RNA的性质
Biochemistry. 1963 May-Jun;2:560-6. doi: 10.1021/bi00903a030.
5
A genetic locus for the regulation of ribonucleic acid synthesis.一个用于调节核糖核酸合成的基因位点。
Proc Natl Acad Sci U S A. 1961 Dec 15;47(12):2005-14. doi: 10.1073/pnas.47.12.2005.
6
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J Bacteriol. 1967 Apr;93(4):1316-26. doi: 10.1128/jb.93.4.1316-1326.1967.
7
Enzymatic thiolation of E. coli sRNA.大肠杆菌小RNA的酶促硫醇化作用
Proc Natl Acad Sci U S A. 1966 May;55(5):1169-74. doi: 10.1073/pnas.55.5.1169.
8
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Proc Natl Acad Sci U S A. 1966 May;55(5):1161-8. doi: 10.1073/pnas.55.5.1161.
9
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Proc Natl Acad Sci U S A. 1965 May;53(5):979-86. doi: 10.1073/pnas.53.5.979.
10
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