L-丝氨酸异羟肟酸抗代谢作用的生化基础。
Biochemical bases for the antimetabolite action of L-serine hydroxamate.
作者信息
Tosa T, Pizer L I
出版信息
J Bacteriol. 1971 Jun;106(3):972-82. doi: 10.1128/jb.106.3.972-982.1971.
Abstract
The amino acid analogue l-serine hydroxamate, which is bacteriostatic for Escherichia coli, has been shown to inhibit protein synthesis. The antimetabolite is a competitive inhibitor of seryl-transfer ribonucleic acid (tRNA) synthetase with a K(i) value of 30 mum. Mutants resistant to l-serine hydroxamate have been selected, and three were shown to have seryl-tRNA synthetases with increased K(i) values. One mutant contains a 3-phosphoglycerate dehydrogenase which is insensitive to inhibition by l-serine.
摘要
对大肠杆菌具有抑菌作用的氨基酸类似物L-丝氨酸异羟肟酸已被证明可抑制蛋白质合成。这种抗代谢物是丝氨酰转移核糖核酸（tRNA）合成酶的竞争性抑制剂，其抑制常数（K(i)）值为30微摩尔。已筛选出对L-丝氨酸异羟肟酸具有抗性的突变体，其中三个突变体的丝氨酰-tRNA合成酶的K(i)值有所增加。一个突变体含有一种对L-丝氨酸抑制不敏感的3-磷酸甘油酸脱氢酶。
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