Isenberg H D, Kominos S, Siegel M
Appl Microbiol. 1969 Oct;18(4):656-9. doi: 10.1128/am.18.4.656-659.1969.
Numerous selective media, available commercially, act by suppressing "normal" bacterial inhabitants of the intestine while permitting the growth of so-called pathogenic representatives of the family Enterobacteriaceae. This investigation attempts to evaluate the action of Salmonella-Shigella (SS) agar, xylose lysine desoxycholate (XLD) agar, and hektoen enteric (HE) agar. Salmonellae and shigellae, isolated from clinical material, were mixed in various ratios with escherichiae, Klebsiella-Enterobacter-Serratia group bacteria, and members of the tribe Proteeae, also of clinical origin. Several of the mixtures were plated in multiple dilutions on the three media. Stools in preservative were also used for evaluation of the media after the addition of definite numbers of the pathogenic bacteria. Results indicate that SS agar suppresses the shigellae along with the autochthonous members of Enterobacteriaceae. XLD and HE agars readily permit the recovery of shigellae as well as salmonellae. This recovery is not obscured by the higher yield of other species obtained with these media.
市场上有许多选择性培养基，其作用原理是抑制肠道中的“正常”细菌群落，同时允许肠杆菌科所谓的致病代表菌生长。本研究旨在评估沙门氏菌-志贺氏菌（SS）琼脂、木糖赖氨酸脱氧胆酸盐（XLD）琼脂和赫克托恩肠道（HE）琼脂的作用。从临床材料中分离出的沙门氏菌和志贺氏菌与同样来自临床的大肠埃希菌、克雷伯菌-肠杆菌-沙雷菌属细菌以及变形杆菌族成员按不同比例混合。将几种混合物以多种稀释度接种到这三种培养基上。在添加一定数量的病原菌后，还使用保存在防腐剂中的粪便来评估这些培养基。结果表明SS琼脂在抑制志贺氏菌的同时也抑制了肠杆菌科的本土成员。XLD琼脂和HE琼脂能轻易地分离出志贺氏菌和沙门氏菌。这些培养基上其他菌种的较高回收率并未掩盖这种分离效果。
