Taylor W I, Schelhart D
Appl Microbiol. 1968 Sep;16(9):1387-93. doi: 10.1128/am.16.9.1387-1393.1968.
The efficiencies of three enrichment broths and four plating media for isolation of enteric pathogens were compared from 1,117 stool specimens. Direct streaking proved to be inferior to enrichment, detecting only 50% of the salmonellae and 61% of the shigellae. By contrast, Selenite Broth (SF) found 90% of the total salmonellae isolates and 82% of the shigellae isolates. Gram-Negative Broth (GN) found 82% and 85%, respectively, but Tetrathionate found only 60% and 39%. Thus, SF and GN were comparable for both salmonellae and shigellae and significantly better than Tetrathionate Broth for both. The plating media compared were MacConkey (MAC), deoxycholate citrate (DC), xylose lysine deoxycholate (XLD), and xylose lysine Brilliant Green (XLBG) Agars. Of the total salmonellae isolated, XLD produced 94%; XLBG, 71%; MAC, 55%; and DC, only 35%. Of shigellae, XLD found 89%; MAC, 75%; XLBG, 63%; and DC, but 27%. The efficacy of XLD is observed to be almost threefold that of DC. The most successful combination of media for the detection of fecal pathogens was GN or SF enrichment broths streaked to XLD plates. These analyses resulted in the isolation of 118 strains of salmonellae and 33 of shigellae.
比较了三种增菌肉汤和四种平板培养基从1117份粪便标本中分离肠道病原体的效率。直接划线法被证明不如增菌法，仅检测到50%的沙门氏菌和61%的志贺氏菌。相比之下，亚硒酸盐肉汤（SF）检出了90%的沙门氏菌分离株和82%的志贺氏菌分离株。革兰氏阴性肉汤（GN）分别检出82%和85%，但四硫磺酸盐肉汤仅检出60%和39%。因此，SF和GN对沙门氏菌和志贺氏菌的检测效果相当，且均显著优于四硫磺酸盐肉汤。所比较的平板培养基有麦康凯（MAC）琼脂、脱氧胆酸盐柠檬酸盐（DC）琼脂、木糖赖氨酸脱氧胆酸盐（XLD）琼脂和木糖赖氨酸煌绿（XLBG）琼脂。在分离出的沙门氏菌中，XLD琼脂上分离出的占94%；XLBG琼脂上的占71%；MAC琼脂上的占55%；DC琼脂上的仅占35%。在志贺氏菌中，XLD琼脂上检出的占89%；MAC琼脂上的占75%；XLBG琼脂上的占63%；DC琼脂上的占27%。观察到XLD的功效几乎是DC的三倍。检测粪便病原体最成功的培养基组合是将GN或SF增菌肉汤划线接种到XLD平板上。这些分析共分离出118株沙门氏菌和33株志贺氏菌。
