用于从粪便标本中分离沙门氏菌和志贺氏菌的培养基比较。
Comparison of media for isolation of salmonellae and shigellae from fecal specimens.
作者信息
Dunn C, Martin W J
出版信息
Appl Microbiol. 1971 Jul;22(1):17-22. doi: 10.1128/am.22.1.17-22.1971.
Abstract
Five transport media, eight plating media, and three enrichment broth media for the isolation of salmonellae and shigellae were evaluated. Eight laboratories in widely separated regions of the United States participated in this evaluation by submitting 490 fecal specimens in the transport media provided. The results suggest that the newer transport media may not offer any advantage over the use of buffered glycerol-saline in the isolation of these enteric pathogens. Shigellae were best isolated by direct inoculation, whereas salmonellae were isolated in greater numbers after tetrathionate (without Brilliant Green) enrichment with subsequent culturing on the plating medium. The use of a variety of plating media is recommended for the recovery of a larger number of these enteric pathogens.
摘要
对用于分离沙门氏菌和志贺氏菌的五种运送培养基、八种平板培养基和三种增菌肉汤培养基进行了评估。美国分布广泛的八个实验室参与了此次评估，提交了用所提供的运送培养基保存的490份粪便标本。结果表明，在分离这些肠道病原体方面，新型运送培养基可能并不比使用缓冲甘油盐水具有任何优势。志贺氏菌通过直接接种能得到最佳分离效果，而沙门氏菌经四硫磺酸盐（不含煌绿）增菌后，再在平板培养基上培养，可分离出更多数量。建议使用多种平板培养基以分离出更多此类肠道病原体。
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