枯草芽孢杆菌生物素生物合成的遗传学
Genetics of biotin biosynthesis in Bacillus subtilis.
作者信息
Pai C H
出版信息
J Bacteriol. 1975 Jan;121(1):1-8. doi: 10.1128/jb.121.1.1-8.1975.
Abstract
Biotin auxotrophs of Bacillus subtilis were isolated and classified into three groups according to growth requirements, cross-feeding pattern, and biotin precursors excreted into culture supernatant fluids. Mutant genes were mapped by transduction using phage PBS1. All presently identified bio genes were linked to aroG with an order of bio-aroG-argA-leu-1. No linked markers were found to the left of the bio loci.
摘要
枯草芽孢杆菌的生物素营养缺陷型菌株被分离出来，并根据生长需求、交叉喂养模式以及分泌到培养上清液中的生物素前体分为三组。使用噬菌体PBS1通过转导对突变基因进行定位。目前所有已鉴定的bio基因都与aroG连锁，顺序为bio-aroG-argA-leu-1。在bio位点左侧未发现连锁标记。
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