Wax R, Rosenberg E, Kosower N S, Kosower E M
J Bacteriol. 1970 Mar;101(3):1092-3. doi: 10.1128/jb.101.3.1092-1093.1970.
Oxidation of glutathione within Escherichia coli cells by diamide, (CH(3))(2)NCON=NCON(CH(3))(2), stops growth but does not cause cell death. Normal growth rates are resumed after periods which vary in length according to the diamide concentration. Consumption of excess reagent with added glutathione quickly reverses the inhibition. Another thiol-oxidizing agent, azoester, C(6)H(5)N=NCOOCH(3), causes lysis.
二酰胺((CH(3))(2)NCON=NCON(CH(3))(2))可使大肠杆菌细胞内的谷胱甘肽氧化,从而停止细胞生长,但不会导致细胞死亡。在根据二酰胺浓度而长短不一的时间段后,细胞会恢复正常生长速率。添加谷胱甘肽消耗过量试剂可迅速逆转这种抑制作用。另一种硫醇氧化剂偶氮酯(C(6)H(5)N=NCOOCH(3))会导致细胞裂解。