Bhattacharyya P, Wendt L, Whitney E, Silver S
Science. 1970 May 22;168(3934):998-1000. doi: 10.1126/science.168.3934.998.
Colicin E1 blocks proline accumulation by membrane vesicles prepared from wild-type sensitive Escherichia coli. Two classes of mutant cells are unaffected by colicin. Vesicles from colicin-resistant strains are sensitive to colicin E1, whereas vesicles from colicin-tolerant strains are unaffected by colicin El. These results suggest that the colicin E1 receptor is on the cell membrane and that colicin-tolerant strains have altered membranes while colicin-resistant strains have altered cell walls.
大肠杆菌素E1可阻止从野生型敏感大肠杆菌制备的膜囊泡积累脯氨酸。两类突变细胞不受大肠杆菌素影响。来自抗大肠杆菌素菌株的膜囊泡对大肠杆菌素E1敏感,而来自耐大肠杆菌素菌株的膜囊泡不受大肠杆菌素E1影响。这些结果表明,大肠杆菌素E1受体位于细胞膜上,耐大肠杆菌素菌株的膜发生了改变,而抗大肠杆菌素菌株的细胞壁发生了改变。