Brunner D P, Graham G S, Treick R W
Arch Microbiol. 1982 May;131(3):229-34. doi: 10.1007/BF00405884.
Phage and colicin-resistant mutants were derived from Escherichia coli K-12P678. Two classes of phage T6 and colicin K-resistant mutants (genotype tsx) were isolated. Tsx-2 mutants, which demonstrated mucoid growth and increased sensitivities to many antibiotics, became sensitive to colicin K when pretreated with ethylenediaminetetraacetate (EDTA), whereas Tsx-1 mutants did not. Reassociation of EDTA-released material partially restored resistance to colicin K for Tsx-2 mutants. When EDTA-released material from strain P678 was associated with either class of K-resistant mutant, an increase in colicin K sensitivity resulted. Observations suggest that colicin K can act on its target site once it penetrates the cell surface. In addition, results suggest that functional colicin K receptors can be transferred from sensitive to resistant strains, thus conferring colicin sensitivity.
噬菌体和大肠杆菌素抗性突变体源自大肠杆菌K-12P678。分离出了两类噬菌体T6和大肠杆菌素K抗性突变体（基因型tsx）。Tsx-2突变体表现出黏液状生长且对多种抗生素敏感性增加，在用乙二胺四乙酸（EDTA）预处理后对大肠杆菌素K变得敏感，而Tsx-1突变体则不然。EDTA释放的物质重新结合可部分恢复Tsx-2突变体对大肠杆菌素K的抗性。当来自菌株P678的EDTA释放物质与任何一类K抗性突变体结合时，大肠杆菌素K敏感性增加。观察结果表明，大肠杆菌素K一旦穿透细胞表面就能作用于其靶位点。此外，结果表明功能性大肠杆菌素K受体可从敏感菌株转移至抗性菌株，从而赋予对大肠杆菌素的敏感性。
