噬菌体P22在青霉素诱导的鼠伤寒沙门氏菌原生质球中的增殖。
Multiplication of bacteriophage P22 in penicillin-induced spheroplasts of Salmonella typhimurium.
作者信息
Ray R K, Burma D P
出版信息
J Virol. 1970 Jan;5(1):45-50. doi: 10.1128/JVI.5.1.45-50.1970.
The spheroplasts of Salmonella typhimurium (LT2) prepared by treatment with penicillin were capable of adsorbing phage P22 C(1). The normal multiplication of the phage took place, although the burst size was reduced to one-fourth of that in intact cells. Rate of incorporation of (14)C-thymidine into spheroplasts was increased severalfold on phage infection. Multiplication of C(+) also took place, but no lysogeny could be established in spheroplasts. Furthermore, spheroplasts prepared from cells lysogenized with wild-type phage, LT2 (C(+)), and a temperature-inducible C(2) mutant, LT2(tsC(2)), were not inducible. Unlike normal cells, both mitomycin C and actinomycin D interfered with the phage multiplication in spheroplasts. The spheroplast system offers great advantages in the study of the synthesis of nucleic acids and proteins in phage-infected LT2.
用青霉素处理制备的鼠伤寒沙门氏菌（LT2）原生质球能够吸附噬菌体P22 C(1)。噬菌体能够正常增殖，尽管其裂解量减少到完整细胞中的四分之一。噬菌体感染后，（14）C-胸腺嘧啶核苷掺入原生质球的速率增加了几倍。C(+)也能增殖，但在原生质球中无法建立溶原状态。此外，由用野生型噬菌体LT2（C(+)）和温度诱导型C(2)突变体LT2(tsC(2))溶源化的细胞制备的原生质球不能被诱导。与正常细胞不同，丝裂霉素C和放线菌素D都干扰噬菌体在原生质球中的增殖。原生质球系统在研究噬菌体感染的LT2中核酸和蛋白质的合成方面具有很大优势。
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