对从兔网织红细胞的较小和较大核糖体亚颗粒中分离出的核糖核酸二级结构的分光光度研究。
A spectrophotometric study of the secondary structure of ribonucleic acid isolated from the smaller and larger ribosomal subparticles of rabbit reticulocytes.
作者信息
Cox R A
出版信息
Biochem J. 1970 Mar;117(1):101-18. doi: 10.1042/bj1170101.
Abstract
The spectrum of RNA from the smaller and larger subparticles of rabbit reticulocyte ribosomes was studied as a function of pH, ionic strength, urea concentration and temperature. It was inferred that both RNA species form short double-helical segments of not more than about 10 base-pairs in length. Not more than about 70% of the base residues may be located in double-helical segments. RNA from the larger subparticle is richer in guanine and cytosine residues and its secondary structure is the more stable. These conclusions are based on the use of double-helical RNA from virus-like particles and of unfractionated Escherichia coli tRNA as model systems.
摘要
研究了兔网织红细胞核糖体较小和较大亚颗粒的RNA光谱随pH值、离子强度、尿素浓度和温度的变化情况。据推断，这两种RNA物种均形成长度不超过约10个碱基对的短双螺旋片段。不超过约70%的碱基残基可能位于双螺旋片段中。较大亚颗粒的RNA富含鸟嘌呤和胞嘧啶残基，其二级结构更稳定。这些结论基于使用病毒样颗粒的双螺旋RNA和未分级的大肠杆菌tRNA作为模型系统。
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THE ISOLATION, CHARACTERIZATION AND ACID-BASE PROPERTIES OF RIBONUCLEIC ACID FROM RABBIT-RETICULOCYTE RIBOSOMES.兔网织红细胞核糖体核糖核酸的分离、特性鉴定及酸碱性质
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