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1
Separation of donor and recipient bacteria by column chromatography.通过柱色谱法分离供体菌和受体菌。
J Bacteriol. 1970 Aug;103(2):302-4. doi: 10.1128/jb.103.2.302-304.1970.
2
Chromatography of microbial cells using continuous supermacroporous affinity and ion-exchange columns.使用连续超大孔亲和柱和离子交换柱对微生物细胞进行色谱分析。
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Mechanical removal of F pili, type I pili, and flagella from Hfr and RTF donor cells and the kinetics of their reappearance.从高频重组(Hfr)和抗药传递因子(RTF)供体细胞中机械去除F菌毛、I型菌毛和鞭毛及其重新出现的动力学。
J Bacteriol. 1969 Jun;98(3):1294-306. doi: 10.1128/jb.98.3.1294-1306.1969.
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Affinity cryogel monoliths for screening for optimal separation conditions and chromatographic separation of cells.用于筛选最佳分离条件及细胞色谱分离的亲和性冷冻凝胶整体柱。
J Chromatogr A. 2006 Aug 11;1123(2):145-50. doi: 10.1016/j.chroma.2006.05.089. Epub 2006 Jul 18.
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[Adsorption condition optimization for anti-HBsAg Fab fragment separation and purification from E. coli using Streamline SP].[利用Streamline SP从大肠杆菌中分离纯化抗-HBsAg Fab片段的吸附条件优化]
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Utilization of anion-exchange chromatography and monoclonal antibodies to characterize multiple pilus types on a uropathogenic Escherichia coli O6 isolate.利用阴离子交换色谱法和单克隆抗体对一株尿路致病性大肠杆菌O6分离株上的多种菌毛类型进行表征。
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Purification of pili from Escherichia coli and Salmonella typhimurium. A preliminary report.
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Improved immunoadsorption procedure with anion-exchange bacterial cell columns.采用阴离子交换细菌细胞柱改进免疫吸附程序。
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Purification of Escherichia coli alkaline phosphatase on an ion-exchange high-performance liquid chromatographic column using carboxymethyl dextrans.使用羧甲基葡聚糖在离子交换高效液相色谱柱上纯化大肠杆菌碱性磷酸酶。
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本文引用的文献

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INHIBITION BY PERIODATE OF MATING IN ESCHERICHIA COLI K-12.过碘酸盐对大肠杆菌K-12交配的抑制作用。
Proc Natl Acad Sci U S A. 1961 Jan;47(1):86-90. doi: 10.1073/pnas.47.1.86.
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An antigen termed f-plus occurring in F-plus E. coli strains.一种名为f-plus的抗原存在于F+大肠杆菌菌株中。
Acta Pathol Microbiol Scand. 1960;48:37-46.
3
Surface properties correlated with sex compatibility in Escherichia coli.大肠杆菌中与性别兼容性相关的表面特性。
J Gen Microbiol. 1956 Aug;15(1):121-32. doi: 10.1099/00221287-15-1-121.
4
F pilus as f+ antigen.F菌毛作为F+抗原。
J Bacteriol. 1967 Jan;93(1):379-89. doi: 10.1128/jb.93.1.379-389.1967.
5
The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in gram negative bacteria.细菌菌毛的结构、功能、合成及基因控制以及革兰氏阴性菌中DNA和RNA转运的分子模型。
Trans N Y Acad Sci. 1965 Jun;27(8):1003-54. doi: 10.1111/j.2164-0947.1965.tb02342.x.
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The identity of the f+ antigen and the cellular receptor for the RNA phage fr.
Mol Gen Genet. 1967;99(1):109-14. doi: 10.1007/BF00306463.
7
Phylogenetic relationships of drug-resistance factors and other transmissible bacterial plasmids.耐药因子与其他可传播细菌质粒的系统发育关系。
Bacteriol Rev. 1968 Mar;32(1):55-83. doi: 10.1128/br.32.1.55-83.1968.
8
Sex pili and the classification of sex factors in the enterobacteriaceae.性菌毛与肠杆菌科中性因子的分类
Nature. 1967 Oct 28;216(5113):343-6. doi: 10.1038/216343a0.

通过柱色谱法分离供体菌和受体菌。

Separation of donor and recipient bacteria by column chromatography.

作者信息

Zsigray R M, Fulk G E, Lawton W D

出版信息

J Bacteriol. 1970 Aug;103(2):302-4. doi: 10.1128/jb.103.2.302-304.1970.

DOI:10.1128/jb.103.2.302-304.1970
PMID:4914561
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC248079/
Abstract

When donor and recipient strains of Escherichia coli were added to columns containing Cellex-P (a cation-exchange cellulose), more than 80% of the female cells passed through the column but only 11% or less of the male cells were eluted. However, when donor strains were blended before their addition to the column, the majority of these cells were eluted. These results indicated that the filamentous appendages termed F pili (which are removed by blending) were the structures responsible for the adherence of donor cells to the cellulose.

摘要

当将大肠杆菌的供体菌株和受体菌株添加到装有Cellex-P(一种阳离子交换纤维素)的柱中时,超过80%的雌性细胞通过了柱子,但只有11%或更少的雄性细胞被洗脱。然而,当供体菌株在添加到柱之前进行混合时,这些细胞中的大多数被洗脱。这些结果表明,被称为F菌毛的丝状附属物(通过混合去除)是供体细胞与纤维素结合的结构。