Zsigray R M, Fulk G E, Lawton W D
J Bacteriol. 1970 Aug;103(2):302-4. doi: 10.1128/jb.103.2.302-304.1970.
When donor and recipient strains of Escherichia coli were added to columns containing Cellex-P (a cation-exchange cellulose), more than 80% of the female cells passed through the column but only 11% or less of the male cells were eluted. However, when donor strains were blended before their addition to the column, the majority of these cells were eluted. These results indicated that the filamentous appendages termed F pili (which are removed by blending) were the structures responsible for the adherence of donor cells to the cellulose.
当将大肠杆菌的供体菌株和受体菌株添加到装有Cellex-P(一种阳离子交换纤维素)的柱中时,超过80%的雌性细胞通过了柱子,但只有11%或更少的雄性细胞被洗脱。然而,当供体菌株在添加到柱之前进行混合时,这些细胞中的大多数被洗脱。这些结果表明,被称为F菌毛的丝状附属物(通过混合去除)是供体细胞与纤维素结合的结构。