Ishibashi M
J Bacteriol. 1967 Jan;93(1):379-89. doi: 10.1128/jb.93.1.379-389.1967.
Specific aggregate formation of F pili was observed, by electron microscopy, in a mixture of male Escherichia coli (or of isolated F pili) and anti-f(+) serum. Cellular appendages other than F pili never showed such aggregation when mixed with anti-f(+) serum. The f(+) agglutinability of male cells, as well as F piliation, was sensitive to mechanical agitation. The f(+) agglutination was inhibited when appropriate numbers of phage M12, capable of attaching to F pili, were mixed with the male culture before the addition of anti-f(+) serum. Correlation between f(+) agglutinability and the extent of F piliation was observed. It was concluded that the F pilus is the structure of the f(+) antigen and is responsible for f(+) agglutination.
通过电子显微镜观察到，在雄性大肠杆菌（或分离出的F菌毛）与抗f(+)血清的混合物中，F菌毛形成了特定的聚集体。当与抗f(+)血清混合时，除F菌毛外的细胞附属物从未出现过这种聚集现象。雄性细胞的f(+)凝集性以及F菌毛形成对机械搅拌敏感。在加入抗f(+)血清之前，将适量能够附着在F菌毛上的噬菌体M12与雄性培养物混合，f(+)凝集反应受到抑制。观察到f(+)凝集性与F菌毛形成程度之间的相关性。得出结论，F菌毛是f(+)抗原的结构，并且负责f(+)凝集反应。
