肽链终止。V. 释放因子在mRNA终止密码子识别中的作用。
Peptide chain termination. V. The role of release factors in mRNA terminator codon recognition.
作者信息
Scolnick E M, Caskey C T
出版信息
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1235-41. doi: 10.1073/pnas.64.4.1235.
Abstract
The protein release factors, R1 and R2, bind to ribosomes in response to specific terminator codons (R1 to UAA or UAG, R2 to UAA or UGA). In reactions containing ribosomes, the tritiated oligonucleotide UA(3)H(2) is retained on nitrocellulose filters in response to either R1 or R2, and UA[(3)H]G in response to R1. These results indicate that an R.terminator codon.70S ribosome intermediate occurs during terminator codon recognition and suggest that protein release factors R1 and R2 recognize terminator codons.
摘要
蛋白质释放因子R1和R2会响应特定的终止密码子与核糖体结合（R1与UAA或UAG结合，R2与UAA或UGA结合）。在含有核糖体的反应中，经氚标记的寡核苷酸UA(3)H(2)会因R1或R2而保留在硝酸纤维素滤膜上，而UA[(3)H]G则因R1而保留。这些结果表明，在终止密码子识别过程中会出现一种R-终止密码子-70S核糖体中间体，这表明蛋白质释放因子R1和R2能够识别终止密码子。
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