Brooks K, Clark A J
J Virol. 1967 Apr;1(2):283-93. doi: 10.1128/JVI.1.2.283-293.1967.
The behavior of lambda phage in the Rec(-) strain JC-1569 is compared with that in the Rec(+) strain JC-1557. No difference deemed significant was noted in the adsorption rate, latent period, burst size, frequency of lysogenization, and frequency of vegetative phage recombination. The location of the prophage and its mode of insertion in the Rec(-) lysogen of wild-type lambda (lambda(+)) were inferred to be normal from the results of conjugational crosses. Spontaneous and ultraviolet (UV) irradiation induction of lambda(+) were markedly reduced in the Rec(-) lysogen. On the other hand, thermal induction of a mutant lambda (lambdacI857) lysogen of the Rec(-) strain was not reduced and was only slightly affected by UV irradiation. Phage subject to inhibition by lambda immunity failed to multiply in UV-irradiated cells of the Rec(-) lambda(+) lysogen, whereas those not inhibited by this immunity did multiply. It was concluded that the failure of UV to induce lambda(+) in the Rec(-) lysogen was not due to damage to the prophage, but rather to the inability of the irradiated cells to respond by lifting immunity. Preliminary evidence indicates that a single mutation confers recombination deficiency and the inability to lift immunity after UV irradiation. Possible relationships between recombination and the lifting of immunity are enumerated.
将λ噬菌体在Rec(-)菌株JC - 1569中的行为与在Rec(+)菌株JC - 1557中的行为进行了比较。在吸附率、潜伏期、裂解量、溶源化频率和营养噬菌体重组频率方面未发现显著差异。从接合杂交的结果推断，野生型λ（λ(+)）的原噬菌体在Rec(-)溶源菌中的位置及其插入模式是正常的。在Rec(-)溶源菌中，λ(+)的自发诱导和紫外线（UV）诱导明显降低。另一方面，Rec(-)菌株的突变型λ（λcI857）溶源菌的热诱导没有降低，且仅受紫外线照射的轻微影响。受λ免疫抑制的噬菌体在Rec(-)λ(+)溶源菌的紫外线照射细胞中无法繁殖，而那些不受这种免疫抑制的噬菌体则可以繁殖。得出的结论是，紫外线在Rec(-)溶源菌中无法诱导λ(+)，不是由于原噬菌体受到损伤，而是由于受照射的细胞无法通过解除免疫来做出反应。初步证据表明，单一突变导致重组缺陷以及紫外线照射后无法解除免疫。列举了重组与解除免疫之间可能的关系。
