磷酸烯醇丙酮酸磷酸转移酶系统在大肠杆菌转运N-乙酰-D-葡萄糖胺中的作用。
The role of the phosphoenolpyruvate phosphotransferase system in the transport of N-acetyl-D-glucosamine by Escherichia coli.
作者信息
White R J
出版信息
Biochem J. 1970 Jun;118(1):89-92. doi: 10.1042/bj1180089.
Abstract
The properties of an N-acetyl-d-glucosamine-transport system have been studied by following the intracellular accumulation of methyl 2-acetamido-2-deoxy-alpha-d-[1-(14)C]glucoside by Escherichia coli. The same analogue was used to assay phosphoenolpyruvate phosphotransferase activity of toluene-treated cells. Transport and phosphorylation are induced by growth on d-glucosamine or N-acetyl-d-glucosamine. Mutants resistant to N-iodoacetyl-d-glucosamine are defective in the uptake and phosphorylation of the labelled glycoside.
摘要
通过追踪大肠杆菌对甲基2-乙酰氨基-2-脱氧-α-D-[1-(14)C]葡萄糖苷的细胞内积累情况，对N-乙酰-D-葡萄糖胺转运系统的特性进行了研究。使用相同的类似物来测定经甲苯处理的细胞的磷酸烯醇丙酮酸磷酸转移酶活性。在D-葡萄糖胺或N-乙酰-D-葡萄糖胺上生长可诱导转运和磷酸化。对N-碘代乙酰-D-葡萄糖胺具有抗性的突变体在标记糖苷的摄取和磷酸化方面存在缺陷。
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