Van Demark P J, Plackett P
J Bacteriol. 1972 Aug;111(2):454-8. doi: 10.1128/jb.111.2.454-458.1972.
The uptake of (14)C-alpha-methyl-d-glucoside (alphaMG) by washed cells of Mycoplasma strain Y was found to be dependent on the supply of metabolic energy. Glycerol or d-mannose, but not l-lactate, would serve as an energy source. Uptake was inhibited by fluoride, iodoacetate, and arsenate, but not by 2,4-dinitrophenol. d-Glucose was inhibitory, presumably by competing for the transport system. The initial product of accumulation had the properties of a phosphate ester of alphaMG. The proportion of free alphaMG in the cells increased with time, until a steady state was reached in which uptake was balanced by the efflux of free alphaMG from the cells. Broken-cell preparations catalyzed a phosphoenolpyruvate-dependent phosphorylation of alphaMG and of d-glucose.
发现支原体菌株Y的洗涤细胞对（14）C-α-甲基-D-葡萄糖苷（αMG）的摄取依赖于代谢能量的供应。甘油或D-甘露糖可作为能量来源，但L-乳酸不能。摄取受到氟化物、碘乙酸盐和砷酸盐的抑制，但不受2,4-二硝基苯酚的抑制。D-葡萄糖具有抑制作用，可能是通过竞争转运系统。积累的初始产物具有αMG磷酸酯的性质。细胞中游离αMG的比例随时间增加，直至达到稳态，此时摄取与游离αMG从细胞中的流出达到平衡。破碎细胞制剂催化了αMG和D-葡萄糖的磷酸烯醇丙酮酸依赖性磷酸化。
