Ivanyi J, Dresser D W
Clin Exp Immunol. 1970 Apr;6(4):493-501.
Improvements have been made in a localized haemolysis in gel replica assay developed previously. The technique is designed to show if an individual antibody producing cell releases antibody of one or more classes. The techniques can also be used to indicate the specificity of antibodies produced by individual cells by using two different antigens in the test system. In experiments where both slides of a slide-pair are untreated or treated identically, and 100% coincidence of plaques is expected, the technique has been shown to detect about 95% coincidence. Pairs of slides treated differently to reveal plaques due to antibodies of different classes show a mean of about 3·5% coincident plaques. This background' of coincidence which it is thought is not due to double-producers', is one of the limiting factors of the technique.
先前开发的凝胶复制品测定法在局部溶血方面已有改进。该技术旨在显示单个产生抗体的细胞是否释放一种或多种类别的抗体。通过在测试系统中使用两种不同的抗原，该技术还可用于指示单个细胞产生的抗体的特异性。在玻片对中的两张玻片均未处理或处理方式相同且预期噬斑100%重合的实验中，该技术已显示能检测到约95%的重合率。为揭示不同类别抗体所致噬斑而进行不同处理的玻片对显示，平均约有3.5%的重合噬斑。这种被认为并非由“双生产者”导致的重合“背景”是该技术的限制因素之一。
