通过凝胶局部溶血（LHG）试验研究抗体产生。3. 产生五种不同类别抗体的小鼠细胞。
Antibody production studied by means of the localized haemolysis in gel (LHG) assay. 3. Mouse cells producing five different classes of antibody.
作者信息
Wortis H H, Dresser D W, Anderson H R
出版信息
Immunology. 1969 Jul;17(1):93-110.
Abstract
By the use of antiglobulin sera of known specificity mouse cells releasing γM, γG, γG, γG or γA antibody to sheep erythrocytes can be identified in the LHG assay. The properties of these sera are described. Cells releasing γG or γG antibody appear as soon after immunization as cells releasing γM antibody. High doses of sheep RBC (1×10) were needed to stimulate the appearance of γA releasing cells in the spleen after intraperitoneal immunization.
摘要
通过使用具有已知特异性的抗球蛋白血清，在淋巴细胞溶血试验（LHG试验）中可鉴定出释放针对绵羊红细胞的γM、γG、γG、γG或γA抗体的小鼠细胞。文中描述了这些血清的特性。释放γG或γG抗体的细胞在免疫后出现的时间与释放γM抗体的细胞一样早。腹腔内免疫后，需要高剂量的绵羊红细胞（1×10）来刺激脾脏中释放γA细胞的出现。
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