Allotype suppression of the responsiveness of adoptively transferred adult spleen cells induced by affinity prepared anti-allotype antibody.

In an adoptive transfer assay it has been shown that a form of allotype suppression can be induced in adult spleen cells. Use of SPF congenic mice, and either anti-allotype serum freed of IgM rheumatoid factor or affinity prepared antibody, has helped to eliminate the possibility of a role for non-specific factors in allotype suppression. Both memory-cell development and a conventional primary response are more susceptible to suppression than memory expression in a secondary response. Non-specific suppressive effects on isotypes other than that of the target allotype (CH) are present but are much smaller than for specific suppression.