大肠杆菌xth突变体中X射线诱导的脱氧核糖核酸单链断裂的修复
Repair of x-ray-induced deoxyribonucleic acid single-strand breaks in xth mutants of Escherichia coli.
作者信息
Seeberg E, Steinum A L
出版信息
J Bacteriol. 1980 Mar;141(3):1424-7. doi: 10.1128/jb.141.3.1424-1427.1980.
Abstract
An exonuclease III-deficient strain of Escherichia coli K-12, BW2001 (xthA11), was unable to perform rapid repair of X-ray-induced deoxyribonucleic acid single-strand breaks and appeared to have a defect in the priming of the 3'-termini necessary for initiation of repair synthesis at the breaks. This defect cannot be explained solely by the lack of exonuclease III activity, because other xth mutants tested, including a deletion mutant, repaired radiation-induced strand breaks at close to the normal rate.
摘要
大肠杆菌K-12的核酸外切酶III缺陷菌株BW2001（xthA11）无法快速修复X射线诱导的脱氧核糖核酸单链断裂，并且在断裂处启动修复合成所需的3'末端引发过程中似乎存在缺陷。这种缺陷不能仅用核酸外切酶III活性的缺乏来解释，因为测试的其他xth突变体，包括一个缺失突变体，以接近正常的速率修复辐射诱导的链断裂。
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