Tomlins R I, Ordal Z J
J Bacteriol. 1971 Feb;105(2):512-8. doi: 10.1128/jb.105.2.512-518.1971.
The heating of Salmonella typhimurium 7136 at 48 C for 30 min produces a population of cells that are incompetent at division on Levine Eosin Methylene Blue Agar containing 2.0% NaCl (EMB-NaCl). When these injured cells were placed in fresh citrate salts medium they recovered, and regained their tolerance to the EMB-NaCl medium and grew out. The addition of the selective inhibitors rifamycin, 5-fluorouracil, 2,4-dinitrophenol, chlorotetracycline, chloramphenicol, and 5-methyl-tryptophan to the recovery medium showed that the recovery process was dependent on ribosomal ribonucleic acid (RNA) synthesis, adenosine triphosphate synthesis, and the synthesis of new protein. These results were substantiated by incorporation experiments, which demonstrated that during recovery no deoxyribonucleic acid synthesis, and hence no cell division, occurred. Ribosomal RNA was synthesized during recovery, but its synthesis was not the rate-limiting step. A small but significant amount of protein was also formed during the latter part of the recovery period.
将鼠伤寒沙门氏菌7136在48℃加热30分钟会产生一群细胞，这些细胞在含有2.0%氯化钠的莱文伊红美蓝琼脂（EMB-NaCl）上无法分裂。当这些受损细胞置于新鲜的柠檬酸盐培养基中时，它们会恢复，并重新获得对EMB-NaCl培养基的耐受性并生长。在恢复培养基中添加选择性抑制剂利福霉素、5-氟尿嘧啶、2,4-二硝基苯酚、金霉素、氯霉素和5-甲基色氨酸表明，恢复过程依赖于核糖体核糖核酸（RNA）合成、三磷酸腺苷合成和新蛋白质的合成。掺入实验证实了这些结果，该实验表明在恢复过程中没有发生脱氧核糖核酸合成，因此也没有细胞分裂。核糖体RNA在恢复过程中合成，但其合成不是限速步骤。在恢复期后期也形成了少量但显著量的蛋白质。
