Gardner P, Harding A L, Olans R N, Smith D H
Appl Microbiol. 1971 Apr;21(4):685-7. doi: 10.1128/am.21.4.685-687.1971.
After overnight incubation of R(+)Escherichia coli with R(-)Salmonella typhimurium in selenite and tetrathionate with Brilliant Green (TBG) broths, R-factor transfer was demonstrated in 10 of 12 experiments. R-factor transfer in these enrichment broths occurred at a markedly reduced frequency in comparison to that in Trypticase soy broth, apparently due to an adverse effect either on the viability of the donor E. coli or the conjugation process itself. Transfer of R factors in commonly used enrichment broths may give rise to falsely resistant antibiotic patterns in Salmonella. However, the frequency of R-factor transfer is so low that it is unlikely to affect significantly the interpretation of R-factor studies.
将R（+）大肠杆菌与R（-）鼠伤寒沙门氏菌在含有煌绿（TBG）的亚硒酸盐和连四硫酸盐肉汤中过夜培养后，在12个实验中有10个证明了R因子的转移。与胰蛋白酶大豆肉汤相比，这些增菌肉汤中R因子的转移频率明显降低，这显然是由于对供体大肠杆菌的活力或接合过程本身产生了不利影响。常用增菌肉汤中R因子的转移可能会导致沙门氏菌出现错误的耐药抗生素模式。然而，R因子转移的频率非常低，不太可能对R因子研究的解释产生显著影响。
