Calderon R A, Hay R J
Immunology. 1984 Nov;53(3):465-72.
An in vivo adoptive cell transfer system was used to investigate protective immunity during experimental murine dermatophytosis due to Trichophyton quinckeanum. Using sublethally (550R) irradiated BALB/c mice which are particularly susceptible to infection, it was found that regional lymph node cells from syngeneic acutely-infected donors conferred protection to irradiated recipients. By contrast, serum from the same donors did not prevent infection of normal BALB/c mice. The capacity of lymphoid cells to transfer adoptive immunity was lost after treatment with monoclonal anti-Thy-1.2 antibody and complement, but not after treatment with monoclonal anti-Ly 2.2 antibody and complement, indicating that cellular immunity is mediated by Ly 2-T cells. The cellular activity of chronically infected mice was also studied. Paradoxically, lymphoid cells from these animals which appeared to be immunologically compromised did, however, confer immunity. Adoptive immunity was abrogated by serum from chronically infected donors or by specific dermatophyte antigen, but not by homologous or heterologous anti-dermatophyte antiserum. These findings indicate that competition between cell-mediated immunity and humoral factors may be the causative mechanism for persistence of dermatophyte infection in this system.
利用体内过继性细胞转移系统，研究了由须发癣菌引起的实验性小鼠皮肤癣菌病期间的保护性免疫。使用对感染特别敏感的经亚致死剂量（550R）照射的BALB/c小鼠，发现来自同基因急性感染供体的局部淋巴结细胞可使受照射的受体获得保护。相比之下，来自相同供体的血清并不能预防正常BALB/c小鼠的感染。用单克隆抗Thy-1.2抗体和补体处理后，淋巴细胞转移过继性免疫的能力丧失，但用单克隆抗Ly 2.2抗体和补体处理后则未丧失，这表明细胞免疫是由Ly 2-T细胞介导的。还研究了慢性感染小鼠的细胞活性。矛盾的是，这些看似免疫受损的动物的淋巴细胞确实能赋予免疫力。过继性免疫被慢性感染供体的血清或特异性皮肤癣菌抗原消除，但同源或异源抗皮肤癣菌抗血清则不能。这些发现表明，细胞介导的免疫与体液因子之间的竞争可能是该系统中皮肤癣菌感染持续存在的致病机制。
