Collotti C, Leskowitz S
J Exp Med. 1970 Mar 1;131(3):571-82. doi: 10.1084/jem.131.3.571.
A number of azobenzenearsonate (ABA) conjugates have been prepared and tested for ability to react with antibody, to sensitize for hapten-specific delayed hypersensitivity and to induce hapten-specific unresponsiveness. All conjugates tested by in vitro or in vivo methods show a capacity to react with preformed antibody. Conjugates of L-amino acid polymers are immunogenic and induce tolerance. Conjugates of D-amino acid polymers or conjugates with high density of ABA groups are nonimmunogenic and fail to induce tolerance. Since paired D- and L-polymer conjugates react comparably with preformed antibody but differ markedly in tolerance induction, it is argued that combination with an antibody-like receptor molecule on the surface of an immune-competent cell is not a sufficient condition for tolerance. The lack of effectiveness of sterically crowded conjugates as well as D-polymer conjugates argues for a preliminary biologic "processing" of antigen necessary for induction of immunity or tolerance. Such a processing event might well involve enzymatic attack on the antigen.
已经制备了多种偶氮苯砷酸盐（ABA）缀合物，并测试了它们与抗体反应、使半抗原特异性迟发型超敏反应致敏以及诱导半抗原特异性无反应性的能力。通过体外或体内方法测试的所有缀合物都显示出与预先形成的抗体反应的能力。L-氨基酸聚合物的缀合物具有免疫原性并诱导耐受性。D-氨基酸聚合物的缀合物或具有高密度ABA基团的缀合物无免疫原性，不能诱导耐受性。由于成对的D-和L-聚合物缀合物与预先形成的抗体反应相当，但在耐受性诱导方面有显著差异，因此有人认为与免疫活性细胞表面的抗体样受体分子结合不是诱导耐受性的充分条件。空间拥挤的缀合物以及D-聚合物缀合物缺乏有效性，这表明诱导免疫或耐受性所需的抗原需要进行初步的生物学“加工”。这样的加工事件很可能涉及对抗原的酶促攻击。
