胃蛋白酶催化转肽作用的途径。反应物种为受体分子阴离子的证据。
The pathway of pepsin-catalysed transpeptidation. Evidence for the reactive species being the anion of the acceptor molecule.
作者信息
Kitson T M, Knowles J R
出版信息
Biochem J. 1971 Apr;122(2):249-56. doi: 10.1042/bj1220249.
Abstract
- The inhibition of pepsin-catalysed hydrolysis of N-acetyl-l-phenylalanyl-l-phenylalanylglycine by the acyl product and product analogues was studied at pH4.3. 2. The acyl product, N-acetyl-l-phenylalanine, gives rise to linear competitive inhibition at pH4.3, whereas at pH2.1 it shows linear non-competitive behaviour. 3. The extent of transpeptidation to N-acetyl-l-[(3)H]phenylalanine during the pepsin-catalysed hydrolysis of N-acetyl-l-phenylalanyl-l-phenylalanyl-glycine is significant at pH4.7, but is undetectable at pH1.3. 4. Both the inhibition and transpeptidation experiments are consistent with the anion of the acceptor molecule being the substrate in pepsin-catalysed transpeptidation. This conclusion supports the formulation of pepsin-catalysed reactions put forward by Knowles et al. (1970).
摘要
- 在pH4.3条件下，研究了酰基产物和产物类似物对胃蛋白酶催化的N-乙酰-L-苯丙氨酰-L-苯丙氨酰甘氨酸水解的抑制作用。2. 酰基产物N-乙酰-L-苯丙氨酸在pH4.3时产生线性竞争性抑制，而在pH2.1时表现出线性非竞争性行为。3. 在胃蛋白酶催化N-乙酰-L-苯丙氨酰-L-苯丙氨酰甘氨酸水解过程中，向N-乙酰-L-[(3)H]苯丙氨酸的转肽程度在pH4.7时显著，但在pH1.3时无法检测到。4. 抑制和转肽实验均与受体分子的阴离子是胃蛋白酶催化转肽反应中的底物这一观点一致。这一结论支持了诺尔斯等人（1970年）提出的胃蛋白酶催化反应的形式。
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Proc Natl Acad Sci U S A. 1961 Jun 15;47(6):759-61. doi: 10.1073/pnas.47.6.759.
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