大肠杆菌中的DNA复制：新掺入的胸腺嘧啶在高分子量DNA分子中的定位。
DNA replication in Escherichia coli: location of recently incorporated thymidine within molecules of high molecular weight DNA.
作者信息
Iyer V N, Lark K G
出版信息
Proc Natl Acad Sci U S A. 1970 Oct;67(2):629-36. doi: 10.1073/pnas.67.2.629.
Abstract
More than 50% of the [(3)H]thymidine incorporated during short pulses into the DNA of Escherichia coli 15T(-) can be extracted by alkali as high molecular weight DNA. Density gradient centrifugation and digestion with exonuclease I suggest that these large pieces of DNA are composed of newly synthesized DNA attached to pre-existing material at the 3' end of the molecule.
摘要
在短脉冲期间掺入大肠杆菌15T(-) DNA中的[(3)H]胸腺嘧啶核苷，超过50% 可以作为高分子量DNA被碱提取出来。密度梯度离心和用核酸外切酶I消化表明，这些大片段DNA是由在分子3' 端连接到预先存在的物质上的新合成DNA组成的。
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